大鼠视神经损伤后Bad蛋白表达与视网膜神经细胞观察

目的观察大鼠视神经钳夹伤后视网膜神经节细胞(RGCs)和Bad蛋白表达变化。方法建立大鼠视神经钳夹伤动物模型,伤后1,3,5,7,9,14,28d处死,HE染色观察RGCs的改变,免疫组化方法检测Bad蛋白的表达水平。结果视神经损伤后1d节细胞开始减少,7d内呈快速减少,14d后呈慢速减少期,28d后趋于稳定;伤后3dBad蛋白表达明显增加,5d时达到高峰,7d后开始下降,14d后变化不明显。结论视神经损伤Bad蛋白的表达诱发RGCs丧失,是伤后视功能下降的重要原因,视功能评价应在损伤28d以后进行。

Changes of retinal ganglion cells and expression of Bad after optic nerve crush in rats

Objective To observe the change of retinal ganglion cells(RGCs)and the expression of Bad after optic nerve injury, so as to study the changes of optic function level on morphology and molecular. Methods The experimental models of optic nerve crush were established in fifty Wistar rats. At the different time after injuries (from one to twenty-eight day), the changes of RGCs were observed under microscope. Immunohistochemiscal technique and computer image analysis methods were performed to observe the changes of Bad in RGCs in rats. Results The number of RGCs was reduced significantly according to partial lesion of optic nerve crush. An initial loss of RGCs densities was accelerated in one week after nerve crush, two weeks later the trend mitigated. After four weeks, no obvious change were observed. The expression of Bad increased in 3 days, reached peak in 5 days, and declined one week later. No obvious changes were observed after two weeks. Conclusion The expression of Bad lead to the loss of RGCs following optic nerve crush. This is the important reason of loss optic function. The identification on optic nerve injuries should be done at least four weeks later.