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| Bcl-2蛋白在外伤性癫痫病灶中的表达 | |
| 引用本文： | 蔡卫林,唐运林,何鹏翔,黄永华,方超,姜富学,竞花兰.Bcl-2蛋白在外伤性癫痫病灶中的表达[J].中国法医学杂志,2010,25(5):312-314,318. |
| 作者姓名： | 蔡卫林 唐运林 何鹏翔 黄永华 方超 姜富学 竞花兰 |
| 作者单位： | 1. 中山大学中山医学院法医学系,广东,广州,510080;中国人民武装警察部队浙江省消防总队医院,浙江,杭州,310011 2. 中国人民解放军第458医院,广东,广州,510000 3. 中山大学中山医学院法医学系,广东,广州,510080 4. 广州市刑事科学与技术研究所,广东,广州,510000 |
| 摘 要： | 目的观察Bcl-2蛋白在外伤性癫痫病灶中的表达变化,探讨其作用机制。方法收集外伤性癫痫、非外性癫痫患者及交通事故死亡者脑皮质各15例,采用免疫组织化学和荧光免疫组织化学技术,观察各实验组Bcl-2蛋白表达水平,测定阳性表达的灰度值,数据采用SPSS 12.0软件进行统计分析。结果外伤性及非外伤性癫痫组Bcl-2蛋白表达水平均高于交通事故死亡组（P〈0.05）;而非外伤性癫痫组表达明显高于外伤性癫痫组,差异亦具有显著统计学意义（P〈0.05）。结论 Bcl-2蛋白能抑制神经元凋亡,对外伤性癫痫的发病过程具有抑制作用。 |
| 关 键 词： | 法医病理学 外伤性癫痫 Bcl-2 免疫组织化学染色 荧光免疫组织化学染色 |
An immunohistochemical study on Bcl-2 in the cerebral focus of posttraumatic epilepsy (PTE) |
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| CAI Weilin,TANG Yunlin,HE Pengxiang,HUANG Yonghua,FANG Chao,JIANG Fuxue,JING Hualan.An immunohistochemical study on Bcl-2 in the cerebral focus of posttraumatic epilepsy (PTE)[J].Chinese Journal of Forensic Medicine,2010,25(5):312-314,318. | |
| Authors: | CAI Weilin TANG Yunlin HE Pengxiang HUANG Yonghua FANG Chao JIANG Fuxue JING Hualan |
| Institution: | 1. Department of Forensic Pathology, Sun Yat-sen University Zhongshan School of Medicine, Guangzhou 510080, China; 2. Guangzhou Criminal Science ＆ Technology Institute, Guangzhou 510080, China;3. No. 458 Hospital of Chinese People＇s Liberation Arm, Guangzhou 510080, China;4. The Fire Fighting Total brigade Hospital of Zhejiang Province of Chinese Armed Police Force, Hangzhou 310011, China) |
| Abstract: | Objective To observe change of Bcl-2 expression in the cerebral focus of posttraumatic epilepsy(PTE) and investigate its pathogenesis.Methods 15 cerebral tissue samples from the PTE patients,15 samples from non-PTE patients and 15 samples from bodies died of traffic accident were collected,respectively.Bcl-2 protein in were observed by immunohistochemical and immunofluorescent techniques.Gray scale of positive immunostaining were measured,and the data were statistically analyzed with SPSS for Windows 12.0.Results Bcl-2 immunoreactivity was stronger in cerebral foci in both PTE and non-PTE patients than in cortical tissues from bodies died of traffic accident.The intensity of Bcl-2 immunoreactivity was significantly stronger in non-PTE patients than in PTE patients(P<0.05).Conclusion The Bcl-2 expression may be up-regulated in the PTE cerebral focus,which may suppress the neuroapoptosis and alleviate the onset of PTE. |
| Keywords: | Bcl-2 |
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