Stability of Morphine,Codeine, and 6‐Acetylmorphine in Blood at Different Sampling and Storage Conditions |
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Authors: | Ioannis Papoutsis PhD Panagiota Nikolaou PhD Constantinos Pistos PhD Artemisia Dona PhD Maria Stefanidou PhD Chara Spiliopoulou PhD Sotirios Athanaselis PhD |
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Institution: | Department of Forensic Medicine and Toxicology, School of Medicine, National and Kapodistrian University of Athens, , Goudi, Athens, 11527 Greece |
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Abstract: | The stability of drugs in biological specimens is a major concern during the evaluation of the toxicological results. The stability of morphine, codeine, and 6‐acetyl‐morphine in blood was studied after different sampling conditions: (i) in glass, polypropylene or polystyrene tubes, (ii) with addition of dipotassium ethylene diamine tetraacetic acid (K2EDTA) or sodium oxalate (Na2C2O4), and (iii) with or without the addition of sodium fluoride (NaF). Spiked blood samples were stored at two different temperatures (4 and ?20°C), analyzed after different storage times and after three freeze–thaw cycles. Opiate concentrations were decreased in all conditions, but the most unstable was 6‐acetyl‐morphine. The addition of NaF as preservative improved the stability of opiates at all conditions studied, whereas the type of anticoagulant did not affect the stability of opiates. It was concluded that blood samples should be stored at ?20°C in glass tubes containing oxalate and NaF for maximum stability. |
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Keywords: | forensic science morphine codeine 6‐acetyl‐morphine stability blood GC/MS |
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