| 超高效液相色谱-串联质谱法同时测定尿液中16种抗生素 |
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| 作者姓名： | Chen C Yan H Shen BH Zhuo XY |
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| 作者单位： | 复旦大学上海医学院法医学系;司法部司法鉴定科学技术研究所上海市法医学重点实验室; |
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| 摘 要： | 目的 建立同时分析尿液中16种抗生素的超高效液相色谱-串联质谱(UPLC-MS、MS)方法.方法 以哌拉西林为内标.尿样中的目标化合物经Oasis HLB固相萃取柱富集、纯化,利用ZORBAX SB-C18色谱柱,以0.1%的甲酸溶液-乙腈为流动相经梯度洗脱分离,采用多反应监测(MRM)模式进行分析.结果各化合物的最低...
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| 关 键 词： | 法医毒理学 抗生素 尿 超高效液相色谱-串联质谱法 |
Simultaneous determination of sixteen antibiotics in human urine with ultra performance liquid chromatography-tandem mass spectrometry |
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| Chen C,Yan H,Shen BH,Zhuo XY.Simultaneous determination of sixteen antibiotics in human urine with ultra performance liquid chromatography-tandem mass spectrometry[J].Journal of Forensic Medicine,2011,27(1):25-29. |
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| Authors: | Chen Cong Yan Hui Shen Bao-hua Zhuo Xian-yi |
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| Institution: | CHEN Cong1,2,YAN Hui2,SHEN Bao-hua2,ZHUO Xian-yi2(1.Department of Forensic Medicine,Shanghai Medical College of Fudan University,Shanghai 200032,China,2.Shanghai Key Laboratory of Forensic Medicine,Institute of Forensic Science,Ministry of Justice,P.R.China,Shanghai 200063,China) |
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| Abstract: | Objective To develop a method for simultaneous determination of sixteen antibiotics in human urine by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods With Piperacillin as an internal standard,the target antibiotics in urine samples were enriched and purified by Oasis HLB solid phase extraction(SPE) cartridges,then separated in a ZORBAX SB-C18 column with a gradient elution of mobile phase of 0.1% formic acid water and acetonitrile,finally analyzed with multiple reaction ... |
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| Keywords: | forensic toxicology antibiotics urine ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) |
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