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Using Frons Width to Differentiate Blow Fly Species (Diptera: Calliphoridae) Phormia regina (Meigen) and Protophormia terraenovae (Robineau‐Desvoidy)
Authors:Sarah V Langer BScFS  Christopher J Kyle PhD  David V Beresford PhD
Institution:1. Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON, Canada;2. Forensic Science Department, Trent University, Peterborough, ON, Canada;3. Biology Department, Trent University, Peterborough, ON, Canada
Abstract:Protophormia terraenovae (Robineau‐Desvoidy) (Diptera: Calliphoridae) and Phormia regina (Meigen) (Diptera: Calliphoridae) are morphologically similar blow fly species commonly used for estimating postmortem intervals. Field collection and storage of adults can result in color changes, in particular on calypters and palps; often collected specimens show damage such as wing fray or fungal growth. We measured the frons width: total head width ratio using photographs (ImageJ version 1.49) to differentiate these two species. Both sexes were distinguishable to species, with the greatest difference between males: 12.34% P. terraenovae versus 1.62% P. regina, less so for females: 40.25% P. terraenovae, versus 33.65% P. regina. Incorporating this feature into future blow fly keys would help with distinguishing field‐caught specimens when other features are obstructed.
Keywords:forensic science  forensic entomology  Calliphoridae  morphology  cytochrome oxidase I gene  species determination
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