| 非目的片段对mtDNA测序影响的实验观察 |
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| 引用本文： | 王新杰,许欣,罗莉静,仪江.非目的片段对mtDNA测序影响的实验观察[J].中国法医学杂志,2009,24(5):325-326. |
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| 作者姓名： | 王新杰 许欣 罗莉静 仪江 |
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| 作者单位： | 潍坊市公安局,山东,潍坊,261061 |
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| 摘 要： | 目的通过重新设计引物，去除非目的片段对mtDNA测序的影响。方法在目的片段原引物内侧重设计引物，用该引物替代原引物进行测序，其测序图谱与原图谱进行对比。结果重新设计引物后，获得了清晰的序列图谱。结论通过重设计引物消除了非目的片断对mtDNA测序的影响。
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| 关 键 词： | 法医物证学 非目的片段 mtDNA测序 |
Experimental observation of the influence of non-target fragments on mtDNA sequence analysis |
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| WANG Xinjie,XU Xin,LUO Lijing,YI Jiang.Experimental observation of the influence of non-target fragments on mtDNA sequence analysis[J].Chinese Journal of Forensic Medicine,2009,24(5):325-326. |
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| Authors: | WANG Xinjie XU Xin LUO Lijing YI Jiang |
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| Institution: | (WANG Xinjie,XU Xin,LUO Lijing,YI Jiang/Weifang Public Security Bureau,Shandong Weifang 261061,China) |
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| Abstract: | Objective To eliminate the influence of non-target DNA fragment on mtDNA sequence analysis by redesigning the primers.Methods Design new primers locating inside of the original ones for mtDNA sequence analysis and compare the latter sequencing electropherograms with original ones.Results Clearer sequencing electropherograms were obtained from PCR products containing the non-target DNA fragments.Conclusion By redesigning the primers,the influence of non-purpose fragments on mtDNA sequence analysis was elimin... |
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| Keywords: | forensic biological evidence non-target DNA fragment mtDNA sequence analysis |
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