弥漫性轴索损伤实验装置的研制及应用

目的自行研制弥漫性轴索损伤的致伤装置,并利用该装置成功复制动物模型。方法采用自行设计的瞬时旋转加-减速致伤装置,使家兔头部在冠状位逆时针加速旋转90°,后瞬间减速运动停止,并于伤后6h、12h、24h3个时相点处死,取材、石蜡包埋,行HE染色、镀银染色及β-APP免疫组化染色,以观测神经轴索的损伤特征。结果致伤后家兔行为和脑组织病理学特征均符合弥漫性轴索损伤表现,大脑中线结构附近（脑皮质下、胼胝体及内囊）出现点灶状出血和斑片状β-APP阳性着色等神经轴索的损伤。结论瞬时旋转加-减速致伤装置可以成功复制弥漫性轴索损伤动物模型,具有操作简单、重复性强的特点,且通过更换不同规格的扭矩弹簧可适用于其他中小型动物。

Designation and application of the DAI experimental facility

Objective To design the DAI experimental facility and establish DAI animal models.Methods With the transiently rotation accelerative and decelerate vulnerate facility designed by author,the heads of rabbits were anticlockwise revolved to 90° and stop fleetly.The rabbits were killed at 6h,12h,24h after injury,respectively.HE staining,silver staining and β-APP immunohistochemistry were done and then the changes of axon were observed.Results After injury,the rabbits,behavior and pathology feature accorded with appearance of DAI.These animals had punctiform hemorrhage and patching positive reaction of β-APP at cortex-alba junction,corpus callosum and internal capsule.Conclusion The vulnerate facility can successfully duplicate the DAI,and the models are easily operated and have a large applicability.