尿中异丙嗪及其代谢物含量硅藻土萃取紫外导数光谱测定法

目的建立尿中异丙嗪原体及其代谢物异丙嗪亚砜的提取测定方法。方法取尿直接倒入硅藻土柱中，用二氯甲烷洗脱，收集洗脱液50℃水浴挥干。剩余物用3．0ml 0．05mol／L硫酸溶解。加入锌粉沸水浴加热，放冷，测定二阶导数光谱。结果异丙嗪亚砜和异丙嗪萃取率均达90％以上，线性范围0．5～5．0μg／ml。结论该方法操作简便，提取率高，重现性好，结果可靠。

Determination of promethazine and its metabolite in urine by solid——phase extraction with celite and UV derivative spectrophotometry

Objective To establish a simple and rapid method for extraction and determination promethazine and its metabolite in urine.Methods The urine was added to celite column and the elutions was effected with dichloromethane.The eluat was evaporated on a water bath at 50℃.The dry residue was redissolved with 3.0ml H2SO4 solution of 0.05mol/L,and then zinc dust was added,in order to make it reaction on the water bath of 100℃ for 3min.The promethazine and its metabolite concentration were determined with the second derivative UV spectrum.Resvlts The extraction rate was 90% for both promethazine and its metabolite.The linear range of determination was 0.5～5.0μg/ml.Conclusion The method for determinating promethazine and its metabolite in urine is simple,convenience,and with high extraction rate.