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| 用斑点ELISA方法检测p30确证人类精斑 | |
| 引用本文： | 张林,李英碧,徐苹蓉,吴梅筠. 用斑点ELISA方法检测p30确证人类精斑[J]. 中国法医学杂志, 1992, 0(2) |
| 作者姓名： | 张林 李英碧 徐苹蓉 吴梅筠 |
| 作者单位： | 华西医科大学法医血清学教研室，华西医科大学法医血清学教研室，华西医科大学法医血清学教研室，华西医科大学法医血清学教研室 成都 610041，成都 610041，成都 610041，成都 610041 |
| 摘 要： | 作者建立了检测精浆特异性抗原p30的斑点ELISA试验方法,确证人类精斑。p30最小检出量为0.4ng。用该法盲测了室温存放1年的生物性斑痕180例,无一例假阳性和假阴性,证明用此法确证人类精斑的敏感性和特异性均优于传统的方法,具有操作简便、快速,检材用量小,结果准确等优点。 |
| 关 键 词： | 斑点ELISA法 精斑确证试验 人类前列腺特异抗原p30 |
DEMONSTRATION OF HUMAN SEMINAL STAINS BY DOT ELISA |
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| Zhang Lin Li Yingbi Xu Pingrong Mu Meiyun DePatmentment of Forensic Serology West China University of Medical Sciences Chengdu. DEMONSTRATION OF HUMAN SEMINAL STAINS BY DOT ELISA[J]. Chinese Journal of Forensic Medicine, 1992, 0(2) | |
| Authors: | Zhang Lin Li Yingbi Xu Pingrong Mu Meiyun DePatmentment of Forensic Serology West China University of Medical Sciences Chengdu |
| Affiliation: | Zhang Lin Li Yingbi Xu Pingrong Mu Meiyun DePatmentment of Forensic Serology West China University of Medical Sciences Chengdu 610041 |
| Abstract: | A new method of anti-p30 Dot ELISA for demonstrating human seminal stain is reported.Using this method,we have done blind test to examin180 biological stains which have been kept at room temperature for more than one year. No false-positive and false-negtive results have been found. The minimal detectable concentration of p30 is 0.4ng. The test results showed that this new method for demonstrating human seminal stains was more sensitive, specific and accurate than other methods. Moreover, this method is easy to perform, not time consuming and have good practical value. |
| Keywords: | Dot Elisa Confirmatory test semen stains Speeific antigen of prost atic gland |
| 本文献已被 CNKI 等数据库收录！ | |