| 7个Y-STR基因座单倍型及其法医学应用 |
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| 作者姓名： | Liu QL Lu DJ Chen LX |
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| 作者单位： | 中山大学中山医学院法医物证教研室,广东,广州,510089 |
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| 摘 要： | 目的调查7个Y-STR基因座单倍型及其法医学应用。方法利用二组复合扩增体系,(Ⅰ:DYS391、GATA-A4、GATA-A10和GATA-H4;Ⅱ:DYS439、DYS437和DYS434)检测7个Y染色体特异性的STR基因座,并用聚丙烯酰胺凝胶电泳和银染显色技术进行基因分型。结果在广东汉族372名无关男性个体中,二组7个基因座分别检出5、7、6、5和6、4、4个等位基因,共检出254种单倍型,其中201种为唯一的。单倍型基因多样性为0.9960。结论7个Y-STR基因座具有很高的识别能力,对建立Y染色体STR数据库,研究群体遗传学和进行法医学鉴定有重要意义。
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| 关 键 词： | Y-STR 单倍型 复合扩增 |
| 文章编号： | 1004-5619(2003)04-0196-03 |
| 修稿时间： | 2003年2月25日 |
Seven Y-specific loci haplotypes and their usefulness in forensic casework |
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| Liu QL,Lu DJ,Chen LX.Seven Y-specific loci haplotypes and their usefulness in forensic casework[J].Journal of Forensic Medicine,2003,19(4):196-198,200. |
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| Authors: | Liu Qiu-ling Lu De-jian Chen Li-xian |
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| Institution: | Faulty of Forensic Medicine, Zhong Shan Medical College, Zhong Shan University, Guangzhou 510089, China. |
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| Abstract: | Objective To study genetic polymorphism of7Y-specific short tandem repeats(STR)and as-sess their usefulness in forensic casework.Methods7Y-STR have been amplified in two multiplex reac-tions,(Multiplex I:DYS391,GATA-A4,GATA-A10and GATA-H4.Multiplex II:DYS439,DYS437and DYS434).PCR products were separated by polyacrylamide gels electrophoresis followed by silver stain.Results When372unrelated individuals from the Han population in Guangdong were detected by those system,DYS391,GATA-A4,GATA-A10,GATA-H4,DYS439,DYS437and DYS434showed5,7,6,5,6,4,4al-leles,respectively.A total of254different haplotypes were identified,of which201(79.13%)were found in single individuals.The overall haplotypes diversity reached0.9960.Conclusion The7Y-STR loci are highly genetic polymorphism and they will be very powerful for establishing Y-STR database,understand-ing human origin,paternity testing and personal identification. |
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| Keywords: | Y-STR haplotypes multiplex PCR |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ |
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