| 用Sep—Pak C18固相萃取柱快速提取净化氨基甲酸酯类农药 |
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| 引用本文： | 刘俊亭,范垂昌.用Sep—Pak C18固相萃取柱快速提取净化氨基甲酸酯类农药[J].中国法医学杂志,1992(4). |
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| 作者姓名： | 刘俊亭 范垂昌 |
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| 作者单位： | 中国医科大学法医系，中国医科大学法医系 沈阳 110001，沈阳 110001 |
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| 摘 要： | 本文介绍了一种应用Sep—Pak C_(18)固相萃取柱从各类生物检材中快速提取净化9种氨基甲酸酯类农药的方法,并用大口径毛细管气相色谱进行分析。9种药物包括:速灭威、叶蝉散、灭多虫、灭除威、灭杀威、残杀威、巴沙、呋喃丹、西维因。萃取前,水基质检材用蒸馏水稀释;脏器检材加0.4N高氯酸溶液。用3ml氯仿/异丙醇(9:1)洗脱药物。水基质检材的回收率(尿、血浆、全血)在80—100％之间;脏器检材的回收率(肝、肾、脑)在66—100％之间。Sep—Pak C_(18)小柱用于尿和血浆样品时可反复使用。
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| 关 键 词： | 氨基甲酸酯类农药 固相萃取Sep—PakC_(18)小柱 气相色谱法 |
RAPID EXTRACTION AND CLEAN-UP OF SOME CARBAMATE INSECTICIDES WITH SEP-PAK C18 SOLIDPHASE EXTRACTION CARTRIDGE |
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| Liu Junting Fan Chuichang Faculty of Forensic Medicine,China Medical University,Shenyang.RAPID EXTRACTION AND CLEAN-UP OF SOME CARBAMATE INSECTICIDES WITH SEP-PAK C18 SOLIDPHASE EXTRACTION CARTRIDGE[J].Chinese Journal of Forensic Medicine,1992(4). |
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| Authors: | Liu Junting Fan Chuichang Faculty of Forensic Medicine China Medical University Shenyang |
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| Institution: | Liu Junting Fan Chuichang Faculty of Forensic Medicine,China Medical University,Shenyang 110001 |
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| Abstract: | A rapid method for isolation and clean-up of 9 carbamate insecticides(MTMC,MIPC,Methomyl,XMC,MPMC,PHC, BPMC, Carbofuran and NAC) with a Sep-Pak C10 solid-phase extraction cartridge from various biological samples, prior to wide-bore capillary gas chromatography,is described.Fluid samples mixed with water and tissue samples mixed with 0.4 N perchloric acid solution were applied to the cartridges, and eluted with 3 ml chloroform/isopropanol (9:1), respectively. Their recoveries were 80-100％ for the fluid samples(urine, plasma and whole blood) and 66-100％) for the tissue samples (liver,kidney and brain).The cartridge could be reused for urine and plasma. |
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| Keywords: | Carbamate insecticides Solid-Phase Extraction Sep-Pak C18 cartri dge Gas chromatography |
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