| HPLC-MS/MS法快速检测人体血液中的夹竹桃毒素 |
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| 引用本文： | 宋蕊,武继锋,刘海燕,栾玉静.HPLC-MS/MS法快速检测人体血液中的夹竹桃毒素[J].中国法医学杂志,2017(5):514-517. |
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| 作者姓名： | 宋蕊 武继锋 刘海燕 栾玉静 |
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| 作者单位： | 1. 济南市公安局刑事科学技术研究所,山东 济南,250002;2. 公安部物证鉴定中心,北京,100038 |
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| 基金项目： | 国家自然科学基金资助项目(81273346) |
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| 摘 要： | 目的采用高效液相色谱-串联质谱法(HPLC-MS/MS)检测人体血液中夹竹桃苷、夹竹桃苷乙。方法采用乙腈沉淀蛋白法处理血液,HPLC-MS/MS法检测,采用MRM记录方式,保留时间和定性离子对定性,标准曲线法定量。结果夹竹桃苷、夹竹桃苷乙的检测限均在0.5ng/m L,线性范围在1ng/m L~1mg/m L,回收率为75.2%~95.7%。结论本方法操作简便,灵敏度高,可应用于中毒案件中人体血液中两种夹竹桃毒素(夹竹桃苷和夹竹桃苷乙)的快速检测。
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| 关 键 词： | 法医毒物分析 高效液相色谱-串联质谱 血液 夹竹桃苷 夹竹桃苷乙 |
Rapid detection of oleandrin and adynerin in human blood by HPLC-MS/MS |
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| Song Rui,Wu Jifeng,Liu Haiyan,Luan Yujing.Rapid detection of oleandrin and adynerin in human blood by HPLC-MS/MS[J].Chinese Journal of Forensic Medicine,2017(5):514-517. |
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| Authors: | Song Rui Wu Jifeng Liu Haiyan Luan Yujing |
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| Abstract: | Objective To establish the analytical method of oleandrin and adynerin in human blood by HPLC-MS/MS. Methods After protein sediment by acetonitril, the concentrations of oleandrin and adynerin in human blood were quantitatively determined by HPLC-MS/MS. The qualitative analysis was conducted based on retention time and MRM ions. Besides, the standard curve method was used for quantification. Results The detection limits of both oleandrin and adynerin were 0.5ng/mL, the linear range was from 1ng/mL to 1mg/mL, with a recovery rate of 75.2%~95.7%. Conclusion The detecting protocol has the advantages of high sensitivity, fast and high accuracy with a relatively wide linear range, which is especially suitable for rapid detection of oleander toxins, alexandrine and adynerin in particular, in human blood in poisoning cases. |
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| Keywords: | forensic toxicological analysis HPLC-MS/MS human blood oleandrin adynerin |
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