应用Miseq FGx平台检测降解骨骼样本效果初探

目的评估Miseq FGx平台检测降解骨骼样本效果。方法对10例基于PCR-CE方法 STR分型结果不理想的降解骨骼样本用Miseq FGx平台检测,比较两个平台STR位点的检出结果和检出率。结果 10例降解骨骼样本在Mi Seq FGx平台上的STR位点检出率均高于PCR-CE方法,一些用PCR-CE方法没有检出的STR位点,很有可能在Miseq FGx平台中检出。同时,Miseq FGx平台测序还获得了X-STR、Y-STR、SNP等遗传标记的大量信息。结论 Mi Seq FGx平台检测降解骨骼样本有较好的应用价值。

A preliminary study of the effects of Miseq FGx sequencing system for degraded bone sample testing

Objective To evaluate the effects of Miseq FGx sequencing system for degraded bone sample testing. Methods Ten degraded bone samples that produced unsatisfactory STR typing with PCR-CE were tested using Miseq FGx, so as to compare the detection result and detection rate of the STR loci between the two systems. Results The detection rates of all the STR loci were higher with MiSeq FGx than with PCR-CE; some of the STR loci that might have not been detectable with PCR-CE were probably detectable with Miseq FGx. Sequencing with Miseq FGx also provided abundant information on the genetic markers including X-STR, Y-STR and SNP. Conclusion MiSeq FGx presents high application value for degraded bone sample testing.