广东广西地区5个群体9个STR基因座的频率调查

目的 调查广东汉族、广西汉族、广西侗族、广西壮族、广西苗族5个群体9个STR基因座多态性,探讨其在法医学检验中的应用价值。方法 应用AmpFISTR Profiler PlusTM荧光标记复合扩增系统,对广东广西5个群体4个民族的1191个无关个体的血样DNA进行9个STR基因座的复合扩增;用ABI 3100遗传分析仪对扩增产物进行分型,统计9个STR基因座的群体遗传学参数。结果 9个STR基因座在广东广西地区5个群体中的累积偶合率为1.51×10-11～8.08×10-11,累积非父排除率为0.99981—0.99990。,结论 该9个STR基因座可满足汉族、壮族、侗族、苗族群体法医学的个体识别及亲权鉴定的需要。

A genetic study of 9 STR loci in 5 populations living in Guangdong and Guangxi provinces

Objective To investigate the genetic polymorphisms of 9 STR loci in Guangdong Han population, Guangxi Han population, Dong population, Zhuang population, and Miao population. Method DNA samples from 328 unrelated individuals in Guangdong Han population and Guangxi Han Population, 199 Zhuang popultion, 162 Dong population, 194 Miao population were screened by using AmpFISTR Profiler PlusTM PCR Amplification Kit and 3100 Genetic Analyzer. Results The genotype frequencies of these 9 STR loci meet the Hardy - Weinberg expectations.The matching probabilities of these 9 STR loci were between 1.51 x 10-11- 8.08 x 10-11 .The cumulative exclusion chances of paternity were 0.99981 - 0.99990. Conclusion Our results showed that the The Profiler Plus?PCR Amplification systems of 9 STR loci are useful in forensic case work in Guangdong Han population, Guangxi Han population, Dong population,Zhuang population and Miao population.