PEP-PCR法及其在法医学中应用的可行性研究

提高微量检材的利用率 ,建立PEP方法并对其法医学应用进行可行性研究。用 15个随机寡核苷酸的序列作为引物 ,低特异性下扩增出微量DNA ,以此扩增物作为模扳 ,进行特异基因座扩增。经PMCT118,GABAR ,DYS19,D18S849,D3S1744,D12S10 90 ,D8S1179,D2 1S11,D18S5 1,D5S818,D13S3 17,D7S82 0 ,D3S13 5 8,vWA ,FGA ,TH0 1,CSF1PO ,TPOX ,D16S5 3 9及Amelogene等 2 0个基因座的PEP PCR与直接的PCR分析比较 ,二者分型结果一致 ,即使 1ngDNA也能得到正确分型。PEP方法可用于法医学检验 ,有利于微量物证的利用 ,使物证检材提供尽可能多的信息

PEP-PCR method and study of reliability of its application in forensic science

We have established a method of primer extension preamplification (PEP) method and studied its practicability 15 oligonucleotides were selected as PEP primer and was amplified under low strength,followed by specifically amplification with loci specific primers Through PEP PCR,about 75% of whole human genomic DNA could be obtained. The results of PCR analyses of 20 loci from PEP products were consistent with those of PCR analyses from the original DNA samples Good results can be obtained from 1ng template DNA It showed that the method of PEP PCR can be applied in the forensic science practice It is important for the increasing usage of the trace evidence and providing nuclei genetic information by PEP PCR method