| 脱落毛发及毛干DNA的STR分型研究 |
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| 引用本文： | 凃政,陈松,李万水,葛芸英,郝金萍,赵,石屹. 脱落毛发及毛干DNA的STR分型研究[J]. 刑事技术, 2011, 0(5): 3-7 |
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| 作者姓名： | 凃政 陈松 李万水 葛芸英 郝金萍 赵 石屹 |
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| 作者单位： | 公安部物证鉴定中心,北京100038;法医遗传学公安部重点实验室,北京100038 |
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| 摘 要： | 目的探讨脱落毛发及毛干细胞核DNA提取、含量和STR分型问题。方法对脱落毛发或毛干进行DNA提取和定量，使用低扩增体系、增加循环次数和多次平行扩增等方法扩增DNA样本，采用叠加比较的方法分析STR分型。结果15cm脱落毛发样品DNA含量大于0．3ng的样品占52．8％，STR分型成功率为55．6％；15cm毛干样品DNA含量大于0．3ng的样品占30．6％，STR分型成功率为25％。结论采用增加循环次数、多次平行扩增等LCN—STR分型方法和Mini—STR试剂盒有助于脱落毛发及毛千的STR基因座分型获得。
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| 关 键 词： | 自然脱落毛发 毛干 DNA提取 污染 STR LCN 细胞核DNA |
STR genotyping of tolegen hairs and hair shafts |
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| TU Zheng,CHEN Song,LI Wan-shui,et al. STR genotyping of tolegen hairs and hair shafts[J]. Forensic Science and Technology, 2011, 0(5): 3-7 |
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| Authors: | TU Zheng CHEN Song LI Wan-shui et al |
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| Affiliation: | TU Zheng,CHEN Song,LI Wan-shui,et al(Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China) |
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| Abstract: | Objective To explore the STR genotyping of tolegen hairs and hair shafts.Methods Nuclear DNA of tolegen hairs or hair shafts was extracted using QIAquick method,quantified using real-time PCR,genotyped using low volume,increasing cycle number and multiple amplifications.Results For tolegen hairs of 15cm,DNA quantity more than 0.3ng was 52.8%,success rate of STR genotyping is 55.6%;for hair shafts of 15cm,DNA quantity more than 0.3ng was 30.6%,success rate of STR genotyping was 25%.Conclusion LCN-STR and Min... |
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| Keywords: | telogen hair shaft extracion contamination short tandem repeat(STR) low copy number(LCN) |
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