基于mTOR/Beclin1通路探讨血竭散对克罗恩病大鼠结肠组织自噬和炎症小体的影响

目的 研究血竭散对克罗恩病大鼠结肠组织自噬和炎症小体的影响,探讨其治疗克罗恩病的作用机制.方法 采用2,4,6-三硝基苯磺酸诱导复制克罗恩病大鼠模型.模型复制后,将36只大鼠随机分为空白组,模型组,血竭散低、中、高剂量组和柳氮磺砒啶(sulfasalazine,SASP)组,每组6只.苏木精-伊红染色法观察大鼠结肠组织...

Effect of Xuejie Powder on Autophagy and Inflammasomes in Colon Tissue of Rats with Crohn's Disease Based on the mTOR/Beclin1 Pathway

Objective To investigate the mechanism of action of Xuejie Powder in the treatment of Crohn's disease(CD)by analyzing its effect on autophagy and inflammasomes in colon tissue of CD rats.Methods After a rat model of CD was established by 2,4,6-trinitrobenzene sulfonic acid,36 rats were randomly divided into control group,model group,sulfasalazine(SASP)group,and low-,middle-,and high-dose Xuejie Powder groups,with 6 rats in each group.Hematoxylin and eosin staining was used to observe the histopathological changes of colon tissue;ELISA was used to measure the expression of inflammatory cytokines in colon tissue;quantitative real-time PCR was used to measure the mRNA expression of Beclin1,NOD-like receptor family pyrin domain containing 3(NLRP3),and absent in melanoma 2(AIM2),and Western blotting was used to measure the protein expression of mammalian target of rapamycin(mTOR),p-mTOR,Beclin1,NLRP3,AIM2,ASC,and Caspase-1;immunofluorescence assay was used to measure the expression of microtubule-associated protein light chain 3B(LC3B).Results Compared with the model group,the Xuejie Powder groups and the SASP group had significant reductions in the histopathological score of colon tissue and the expression levels of interleukin-1β,interleukin-18,and tumor necrosis factor-α(P<0.05).Compared with the control group,the model group had a significant reduction in the expression level of p-mTOR in colon tissue and significant increases in the expression levels of Beclin1,LC3B,NLRP3,AIM2,ASC,and Caspase-1(P<0.05).Xuejie Powder and SASP upregulated the expression level of p-mTOR and downregulated the expression levels of Beclin1,LC3B,NLRP3,AIM2,ASC,and Caspase-1(P<0.05).Conclusion Xuejie Powder might inhibit intestinal cell autophagy,reduce the activation of inflammasomes,and improve colitis in CD rats by regulating the mTOR/Beclin1 signaling pathway.