顶空进样-气相色谱-质谱法测定芪白平肺胶囊中挥发性成分

目的 测定芪白平肺胶囊中挥发性成分。方法 采用顶空进样-气相色谱-质谱联用技术，并通过所用质谱库检索研究其挥发性化学成分组成，用面积归一化法确定各成分的相对百分含量。结果 共分辨出68个色谱峰，鉴定出53个化学成分，约占总挥发性成分含量的90.75%，主要挥发性成分为酯类(38.58%)、酸类(22.97%)、杂环类(13.54%)、醛类(8.75%)、醇类(2.84%)、醚类(2.71%)、芳烃类(0.74%)和酮类(0.62%)物质，其中含量最高的是乙酸乙酯(25.54%)，其次为乙酸(11.79%)，含量较高的为2-甲基丁醛(7.00%)，2-氨基丁酸(6.83%)，乙酸丙酯(5.60%)，2，3-二羟基丙酸(3.45%)，糠醛(3.08%)，5-甲基糠醛(3.05%)，2-甲基四氢呋喃-3-酮(2.82%)，2-乙酰基呋喃(2.54%)，甲基环氧丙烷(2.03%)，等。结论 所建立的方法分离效果较好，能够准确鉴定芪白平肺胶囊中主要挥发性成分。

Determination of Volatile Components in Qibai Pingfei Capsule by Headspace-Gas Chromatography-Mass Spectrometry

Objective To determine the volatile components in Qibai Pingfei Capsule. Methods The volatile components in Qibai Pingfei Capsule were identified by headspace-gas chromatography-mass spectrometry and NIST Mass Spectral Database, and the content of each component was determined by area normalization. Results Sixty-eight chromatographic peaks were obtained, and 53 compounds were identified, accounting for 90.75% of all volatile components. The main volatile components were ester (38.58%), acid (22.97%), heterocycle (13.54%), aldehyde (8.75%), alcohol (2.84%), ether (2.71%), arene (0.74%), and ketone (0.62%). Ethyl acetate had the highest content (25.54%), followed by acetic acid (11.79%), 2-methylbutyraldehyde (7.00%), 2-aminobutyric acid (6.83%), propyl acetate (5.60%), 2,3-dihydroxypropanoic acid (3.45%), furaldehyde (3.08%), 5-methylfurfural (3.05%), 2-methyltetrahydrofuran-3-one (2.82%), 2-acetylfuran (2.54%), and isobutylene epoxide (2.03%). Conclusion With the established method, the main volatile components in Qibai Pingfei Capsule can be well separated and accurately identified.