基于RhoA/ROCK通路研究天麻素对高血压病左心室肥厚大鼠心室重构的影响 |
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作者姓名: | 侯玮琼 姜北 赵岩 杨勋超 毛文超 吴欣芳 |
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作者单位: | 中国中医科学院广安门医院南区内一科,北京 102618;中国中医科学院广安门医院南区内一科,北京 102618;中国中医科学院广安门医院南区内一科,北京 102618;中国中医科学院广安门医院南区内一科,北京 102618;中国中医科学院广安门医院南区内一科,北京 102618;中国中医科学院广安门医院南区内一科,北京 102618 |
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基金项目: | 中国中医科学院广安门医院南区院级科研基金项目(Y2014-06) |
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摘 要: | 目的 观察天麻素对高血压病左心室肥厚大鼠心室重构的影响,从Ras同族体基因家族成员A(Ras homolog gene family member A,RhoA)/Rho激酶(Rho-associated kinase,ROCK)通路探究其作用机制.方法 将100只SD雄性大鼠随机分为假手术组,模型组,天麻素低剂量(1...
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关 键 词: | 天麻素 左心室肥厚 信号通路 RhoA ROCA 心室重构 |
Effect of Gastrodin on Ventricular Remodeling in Hypertensive Rats with Left Ventricular Hypertrophy: A Study Based on the RhoA/ROCK Pathway |
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Authors: | HOU Wei-qiong JIANG Bei ZHAO Yan YANG Xun-chao MAO Wen-chao WU Xin-fang |
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Institution: | First Department of Internal Medicine, Southern Branch of Guang''anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 102618, China |
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Abstract: | Objective To investigate the effect of gastrodin on ventricular remodeling in hypertensive rats with left ventricular hypertrophy(LVH)and its mechanism of action based on the Ras homolog gene family,member A(RhoA)/Rho-associated kinase(ROCK)pathway.Methods A total of 100 male Sprague-Dawley rats were randomly divided into sham-operation group,model group,low-,middle-,and high-dose gastrodin groups(at doses of 16,24,and 32 mg/kg,respectively),and enalapril group(1.5 mg/kg),with 15 rats in each group.A rat model of LVH was established by the abdominal aorta constriction method.ELISA was used to measure the serum levels of angiotensinⅡ(AngⅡ)and interleukin-6(IL-6);left ventricular hypertrophy index(LVHI)was calculated;hematoxylin and eosin staining and Masson staining were used to observe histomorphological changes of the myocardium;qRT-PCR and Western blot were used to measure the mRNA and protein expression of RhoA and ROCK in myocardial tissue.Results Compared with the sham-operation group,the model group had significant increases in systolic pressure of the caudal artery,LVHI,and serum levels of AngⅡand IL-6(P<0.05),a higher degree of myocardial fibrosis,and significant increases in the mRNA and protein expression levels of RhoA and ROCK(P<0.05).Compared with the model group,the low-,middle-,and high-dose gastrodin groups and the enalapril group had significant reductions in systolic pressure of the caudal artery,LVHI,and serum levels of AngⅡand IL-6(P<0.05),a lower degree of myocardial fibrosis,and significant reductions in the mRNA and protein expression levels of RhoA and ROCK(P<0.05).The enalapril group had the lowest mRNA and protein expression levels of RhoA and ROCK in myocardial tissue,and gastrodin exerted an effect in a dose-dependent manner.Conclusion Gastrodin can effectively reduce blood pressure of the caudal artery,LVHI,and serum levels of AngⅡand IL-6 and improve ventricular remodeling in hypertensive rats,possibly by inhibiting the RhoA/ROCK signaling pathway. |
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Keywords: | Gastrodin Left ventricular hypertrophy Signaling pathway Ras homolog gene family member A Rho-associated kinase Ventricular remodeling |
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