高效液相色谱法测定桔梗多糖对桔梗皂苷D在大鼠体内药物代谢动力学参数的影响

目的 考察桔梗多糖(Platycodon grandiflorum polysaccharides,PGP)对桔梗皂苷D(platycod-in D,PD)在大鼠体内药物代谢动力学参数的影响,为桔梗的临床应用提供实验依据.方法 将24只SD大鼠随机分为PD组,PD+PGP低、中、高剂量组(PD-PGP-L、PD-PGP-M、PD-PGP-H组),灌胃给药后在各时间点眼眦取血,采用高效液相色谱法测定给药后PD的血药浓度变化,由DAS 2.1软件拟合药物代谢动力学参数.结果 与PD组比较,各PD-PGP实验组均显著缩短PD的达峰时间(P<0.05),并显著提高峰浓度(P<0.05)以及药时曲线下面积(P<0.05).结论 PGP能够显著改变PD口服后的药物代谢动力学参数.

Effect of Platycodon grandiflorum Polysaccharides on the Pharmacokinetic Parameters of Platycodin-D in Rats: An Analysis by High-performance Liquid Chromatography

Objective To investigate the effect of Platycodon grandiflorum polysaccharides (PGP) on the pharmacokinetic parameters of platycodin-D (PD) in rats and to provide experimental data for clinical application of Platycodon grandiflorum. Methods Twenty-four Sprague-Dawley rats were randomly divided into PD group and PD+PGP groups with low, medium, and high doses of PGP (PD-PGP-L, PD-PGP-M, and PD-PGP-H groups). After oral administration, blood was taken from the eye canthus at each time point, and the plasma concentration of PD was determined by high-performance liquid chromatography. Pharmacokinetic parameters were fitted using DAS 2.1 software. Results Compared with the PD group, PD in the PD-PGP-L, PD-PGP-M, and PD-PGP-H groups had significantly reduced time to peak (P<0.05) and significantly increased peak concentration (P<0.05) and area under the concentration-time curve (P<0.05).Conclusion PGP can significantly change the pharmacokinetic parameters of PD after oral administration.