构树皮总黄酮提取及抗菌活性研究

目的 提取构树皮有效的抑菌活性部位。方法 采用正交试验法优化构树皮黄酮最佳超声提取工艺，并采用AB-8大孔吸附树脂富集构树皮总黄酮提取物活性部位，用平皿药基法比较不同部位的抑菌效果。结果 构树皮总黄酮的最佳提取工艺为：以10倍体积的70%乙醇为溶剂，超声提取20 min，功率300 W。经AB-8大孔吸附树脂富集分离后，70%乙醇洗脱部位对浅部真菌红色毛癣菌和白色念珠菌均有良好的抑制作用。结论 所采用的工艺操作简单、耗能低、总黄酮提取率高，抑菌作用强。

Extraction and Antimicrobial Activity of Total Flavonoids from Cortex of Broussonetia papyrifera

Objective To extract the potentially antimicrobial components from cortex of Broussonetia papyrifera. Methods The optimization of ultrasonic extraction of flavonoids from cortex of Broussonetia papyrifera was performed using the orthogonal design. The active components of flavonoids extract from cortex of Broussonetia papyrifera were enriched using AB-8 macroporous resin. Comparison of antimicrobial effects between different components was performed using drug-containing culture medium. Results The optimal extraction of total flavonoids from cortex of Broussonetia papyrifera was performed by 20-min ultrasonic extraction (300 W) using 10 volumes of 70% ethanol as a solvent. After enrichment and separation using AB-8 macroporous resin, the 70% ethanol elution fraction had good inhibitory effects on superficial fungi including Trichophyton rubrum and Candida albicans. Conclusion This extraction technique features simple operation, low power consumption, high extraction rate of total flavonoids, and potent antimicrobial activity of antimicrobial components.