Abstract: | Objective To comparatively analyze the volatile components in Panax notoginseng extracted with different methods by gas chromatography-mass spectrometry (GC-MS).Methods The volatile components in Panax notoginseng were extracted by reflux extraction, ultrasonic extraction, and static headspace extraction. The volatile components were identified by GC-MS and NIST mass spectral database (11.0), and the relative content of these compounds was determined by peak area normalization. Results Sixty, fifty, and twenty-four constituents were identified from the volatile compounds extracted through reflux extraction, ultrasonic extraction, and static headspace extraction, respectively. Among all the volatile components extracted by static headspace extraction, sabinene was the most predominant component, accounting for 24.97% of the total. Among all the volatile components extracted by reflux extraction and ultrasonic extraction, oxacyclotetradeca-4,11-diyne was the most predominant component, accounting for 9.97% and 14.75%, respectively, of the total. Three polyacetylenes and two steroids were identified from the volatile components of Panax notoginseng for the first time. Conclusion The main components of volatile components include alkanes, olefins, aldehydes, acids, esters, terpenes, polyacetylenes, steroids, and heterocyclic compounds. The volatile components in Panax notoginseng can be extracted fully by taking advantage of three different methods. |