| 中国人p33.6位点的扩增片段长度多态性 |
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| 引用本文： | 陈松,李伯龄,倪锦堂,胡兰,季安全,叶健.中国人p33.6位点的扩增片段长度多态性[J].中国法医学杂志,1993(3). |
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| 作者姓名： | 陈松 李伯龄 倪锦堂 胡兰 季安全 叶健 |
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| 作者单位： | 公安部第二研究所，公安部第二研究所，公安部第二研究所，公安部第二研究所，公安部第二研究所，公安部第二研究所 北京 100038，北京 100038，北京 100038，北京 100038，北京 100038，北京 100038 |
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| 摘 要： | 用PCR、小型聚丙烯酰胺凝胶电泳和银染法对小卫星区域p33.6(D1S111)位点的扩增片段长度多态性(Amp—FLP)进行分析和对100例无关中国人p33.6位点的等位基因频率进行调查及数据处理,发现该位点核心序列重复数从9到22之间的全部14个等位基因,片段长度分布于435～925bp之间,基因频率为0.5～35.5％,杂合度为76％。对6个家系共22名相关个体进行分析,符合孟德尔遗传定律;对人体各种不同组织DNA进行该位点的分析,显示出高度的一致性。该位点适用于法医学上的个人识别以及亲子鉴定。
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| 关 键 词： | p33.6位点 多聚酶链反应(PCR) 可变数目的串联重复(VNTR) 扩增片段长度多态性(Amp—FLP) |
AMPLIFIED FRAGMENT LENGTH POLYMORPHISMS ANALYSIS OF P33.6 LOCUS IN CHINESE |
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| Chen Song Li Boring Ni Jintang Hu Lan Ji Anquan Ye Jian Institute of Forensic Science,Ministry of Public Security,Beijing.AMPLIFIED FRAGMENT LENGTH POLYMORPHISMS ANALYSIS OF P33.6 LOCUS IN CHINESE[J].Chinese Journal of Forensic Medicine,1993(3). |
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| Authors: | Chen Song Li Boring Ni Jintang Hu Lan Ji Anquan Ye Jian Institute of Forensic Science Ministry of Public Security Beijing |
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| Abstract: | The polymorphisms of minisatellite p33.6 (D1S111) locus were typed rapidly and accurately us- ing polymerase chain reaction (PCR). The amplified fragments were analyzed by mini- polyacrylamide gel electrophoresis followed by silver stain. The distribution of allele frequencies among 100 unrelated Chinese is reported. 14 alleles, containing 9 to 22 repeat units, had been detected with a heterozygosity of 76%. The allele frenquencies were from 0.5 %to 35.5 %. The size of amplified fragments ranged from 435 bp to 925bp. The discrimination power of p33.6 locus was 0. 916. The Amp --FLP was inherited according to the Mendelian Law. The results showed that the polymorphisms of p33.6 locus can be used for individual identification and paternity test. |
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| Keywords: | p33 6 locus Polymerase Chain Reaction(PCR) Variable Number of Tandem Repeat(VNTR) Amplified Fragment Length Polymorphism(Amp—FLP) |
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