| 不同分型方法的STR分型差异 |
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| 引用本文： | 吕德坚,陈玉川,陆惠玲.不同分型方法的STR分型差异[J].中国法医学杂志,2002,17(1):14-16. |
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| 作者姓名： | 吕德坚 陈玉川 陆惠玲 |
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| 作者单位： | 中山大学中山医学院法医物证教研室,广东,广州,510089 |
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| 摘 要： | 目的调查不同的STR分型系统之间分型的一致性。方法 10 0例不同个体的DNA样本分别用单位点聚丙烯酰胺凝胶银染法和PowerPlex16System试剂盒对 13个法医学常用STR位点进行基因分型 ,并比较两种不同分型系统间的分型结果。结果 1例样本在D8S1179位点出现了分型不一致的结果 :银染法的基因型为 12 / 14 ,而用PowerPlex16System试剂盒的分型则为 12 / 15。结论不同的STR分型系统可导致不同的基因分型
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| 关 键 词： | 短串联重复(STR) 基因分型 异常分型 聚合酶链反应(PCR) |
| 文章编号： | 1001-5728(2002)01-0014-03 |
| 修稿时间： | 2001年7月1日 |
STR typing discordance between different typing methods |
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| LU Dejian,CHEN Yuchuan,LU Huiling.Faculty of Forensic Medicine,Sun Yat sen University of Medical Sciences,Guangzhou.STR typing discordance between different typing methods[J].Chinese Journal of Forensic Medicine,2002,17(1):14-16. |
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| Authors: | LU Dejian CHEN Yuchuan LU HuilingFaculty of Forensic Medicine Sun Yat sen University of Medical Sciences Guangzhou |
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| Institution: | LU Dejian,CHEN Yuchuan,LU Huiling.Faculty of Forensic Medicine,Sun Yat sen University of Medical Sciences,Guangzhou 510089 |
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| Abstract: | Objective To investigate the STR typing discordance between different typing methods.Methods Genotypes of 13 routine forensic STR loci in DNA samples from 100 individuals were typed by using singleplex polyacrylimide gel electrophoresis silver stain system and Power Plex16 System,respectively.The typing results between these two systems were compared.Results One genotype discordance was observed at D8S1179 locus in a DNA sample.The genotypes was 12/14 in snigleplex system and 12/15 in Power Plex16 System.Conclusion Different STR typing systems may result in different genotyps from the same DNA sample. |
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| Keywords: | Short tandem repeats(STR) Genotype Anomalous typing Polymerase chain reaction(PCR) |
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