米尔贝肟对犬钩虫的体外作用

为了评价犬钩虫对米尔贝肟的体外敏感性,试验设空白对照组,米尔贝肟高、中、低剂量组及伊维菌素对照组,考察了药物对犬钩蚴三期幼虫形态和活力的影响及对其移行能力的影响。结果显示,未添加药物的钩蚴大部分保持笔直,而几乎没有弯曲或者卷曲,而在药物添加组,部分钩蚴出现大于45°角的弯曲,弯曲程度远远大于未添加药物的正常虫体;米尔贝肟用药量在1×10-5 g/mL时,8h杀虫率达100%,米尔贝肟用药量为1×10-6 g/mL时杀灭犬钩虫幼虫的显效时间及杀虫效果与伊维菌素组差异不显著(P>0.05);米尔贝肟和伊维菌素对犬钩蚴的移行有显著的抑制作用。结果表明,犬钩虫对米尔贝肟在体外具有一定的敏感性。

In vitroanthelmintic sensitivity of milbemycin oxime to Ancylostoma caninum

Anthelmintic sensitivity of milbemycin oxime to Ancylostoma caninum in vitrowas studied.Milbemycin oxime groups with concentrations of 1×10-5,1×10-6,1×10-7,1×10-8 and 1×10-9 g/mL respectively,as well as ivermectin group and control group were adopted to detect effects of milbemycin oxime on the morphology of quiescent larvae,larval motility and larval migration.It showed that most larvae under drug-free conditions assumed a straight morphology,free from deviations,and kinking or coiling.However,in subsequent drug-exposed wells,an increasing proportion of quiescent larvae had major deviations(kinking) at angles of greater than 45°,and these deviations involved a greater portion of the body than those associated with normally quiescent larvae under control conditions.Anthelmintic rate of milbemycin oxime can achieve 100% in the 8th hour at concentration of 1×10-5 g/mL,and milbemycin oxime has the same effects with ivermectin at the concentration of 1×10-6 g/mL(P>0.05).Both milbemycin oxime and ivermectin had significant inhibitory effects on larval motility.In conclusion,Ancylostoma caninum is sensitive to milbemycin oxime.