| 细胞色素b基因序列测定进行种属鉴定应用研究 |
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| 引用本文： | 刘超,刘宏,李红霞,王穗保.细胞色素b基因序列测定进行种属鉴定应用研究[J].刑事技术,2003,200(4):11-13. |
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| 作者姓名： | 刘超 刘宏 李红霞 王穗保 |
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| 作者单位： | 广州市刑事科学技术研究所,广州,510030 |
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| 基金项目： | 广州市重点科技攻关项目(No.98-z-018) |
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| 摘 要： | 目的建立细胞色素b基因序列测定进行种属鉴定的方法。方法应用特异性引物扩增人与14种常见动物细胞色素b基因14817～15174区间长度为358bp的片段,用荧光标记自动测序技术对此片段进行序列测定,比较这些动物与人之间片段的序列差异。结果所有血痕样本均可以得到良好的测序结果,所测14种动物细胞色素b基因358bp的片段序列与人之间均存在明显的差异,最小为20.1%,最大差异为28.5%。结论测定细胞色素b基因序列进行种属鉴定特异性强、适用检材广,具有良好的应用价值。
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| 关 键 词： | 种属鉴定 细胞色素b 序列差异 |
| 文章编号： | 1008-3650(2003)04-0011-03 |
| 修稿时间： | 2002年11月1日 |
Application to species identification by sequencing the cytochrome-b gene specific fragment |
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| LIU Chao,LIU Hong,LI Hong-xia,et al.Application to species identification by sequencing the cytochrome-b gene specific fragment[J].Forensic Science and Technology,2003,200(4):11-13. |
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| Authors: | LIU Chao LIU Hong LI Hong-xia |
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| Abstract: | Objective Identify the samples species by sequencing the cytochrome-b gene fragment.Methods Using the special primer,we can get the PCR products of human and14ordinary kinds animals cytochrome-b gene358bp segment.With fluorescent sequence technique,sequence results of all blood samples were detected.Results Compare the sequence of human cytochrome-b gene segment with the other14different animals,there are obvious difference between the animals and human.The discrepancy according as the statistical in this study is20.1%to28.5%.Conclusion Indentify the species by sequence the cytochrome-b gene segment is a available and exact technique,it can be used in almost all kinds of biological samples. |
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| Keywords: | species identification cytochrome b sequence difference |
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