旋毛虫ES抗原对小鼠巨噬细胞TLR2/4mRNA表达的影响

为探讨旋毛虫ES抗原对RAW264.7细胞TLR2/4mRNA表达的影响,分别取经0、2、5、15、30、45μg/mL ES抗原作用24h的RAW264.7细胞和用15μg/mL ES抗原作用0、3、6、12、18、24h后的RAW264.7细胞,采用半定量PCR方法检测TLR2和TLR4mRNA的表达水平变化。结果显示,随着ES抗原浓度的升高,TLR2/4mRNA的表达量逐渐上升,15μg/mL ES抗原组与空白对照组相比差异显著(P<0.05)。在15μg/mL ES抗原作用24h内,随着作用时间的延长,TLR2/4mRNA的表达量逐渐上升,作用18h后的表达水平升高,且与空白对照组差异显著(P<0.05)。证实,ES抗原可刺激RAW264.7细胞表面受体TLR2/4表达升高,且存在一定的剂量和时间效应。

Effect of Trichinella spiralis excretory-secretory antigen on expression of TLR2/4 mRNA in mouse macrophage

To explore the effect of Trichinella spiralis excretory-secretory antigen on the expression of mRNA of TLR2/4 in RAW264.7 cell,the RAW264.7 cells stimulated respectively by ES antigen(0,2,5,15,30 and 45 g/mL) for 24 h and 15 g/mL ES antigen stimulated for 0,3,6,12,18 and 24 h were collected,and the expression levels of TLR2 mRNA and TLR4 mRNA were detected by RT-PCR.In result,the expression levels of TLR2/4 mRNA in RAW264.7 cell increased followed the ES antigen concentration,and it showed significantly difference at the concentration of 15 g/mL(P<0.05).At the concentration of 15 g/mL,the expression of TLR2/4 mRNA in RAW264.7 cell increased with time,and increased significantly after 18 h(P<0.05).The results showed that ES antigen has impact on the expression of TLR2/4 in RAW264.7 cell,and the expression level is related to dosage and time.