可卡因对性成熟期雄性大鼠生殖功能的损害

目的探讨可卡因对性成熟期雄性大鼠生殖功能的影响及其作用机制。方法选用性成熟期健康雄性SD大鼠30只，随机分为实验组和对照组，每组15只。实验组大鼠以15mg／kg剂量每天皮下注射可卡因28d。观察动物体质量、睾丸质量改变，检测血液中激素含量的变化．利用原位缺口末端标记（TUNEL）法检测睾丸细胞的凋亡，免疫组化方法检测睾丸Fas基因的表达。结果（1）实验组大鼠体质量、睾丸质量明显低于对照组（P〈0．05）；（2）实验组与对照组相比，睾酮含量明显降低（P〈O．05）：（3）实验组睾丸细胞凋亡较对照组明显增多（P〈0．05），Fas基因表达明显增加（P〈0．05）．且Fas基因阳性表达与睾丸细胞的凋亡指数呈正相关（r=0．9012，P〈O．05）。结论可卡因可致大鼠生殖器官发育和生殖内分泌功能损害．造成生精细胞凋亡．增殖能力下降．可能与Fas介导的睾丸生精细胞凋亡机制有关。

The Reproductive System Impairment of Adult Male Rats Induced by Cocaine

Objective To investigate the reproductive system impairment induced by cocaine in adult male rats and the possible underlying mechanism. Methods Thirty adult male rats were randomly divided into experimental and control groups, with 15 rats in each groug. Rats of the experimental group were injected cocaine hydrochloride（15mg/kg body weight） subcutaneously daily for four weeks. The weight of body and testis, as well as the level of serum hormone of the rats were examined. In addition, the apoptosis rate of testicular tissue by TUNEL and the expression of Fas gene in testicular tissue were examined by im- munohistochemistry. Results Compared with the control, the weight of testis in the cocaine exposed group decreased significantly （P〈0.05）, and the serum testosterone level decreased significantly（P〈0.05）. Moreover, both the apoptosis rate and the expression of Fas gene increased in the testieular tissue of rats in the cocaine exposed group in comparison to the control group（P〈0.05）. The apoptosis rate was significantly correlated with the expression of Fas gene（r=0.9012, P〈O.05）. Conclusion Cocaine may cause reproductive system injury in adult male rats, and Fas-mediated apoptosis may be one of the functional mechanisms involved in the reproductive system injuried by cocaine.