| 植物酶在解剖组织中有机磷、氨基甲酸酯农药检测中的应用 |
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| 引用本文： | 刘久华,王清福,施昆.植物酶在解剖组织中有机磷、氨基甲酸酯农药检测中的应用[J].中国法医学杂志,1989(2). |
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| 作者姓名： | 刘久华 王清福 施昆 |
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| 作者单位： | 河北省公安厅，河北省公安厅，河北省公安厅 |
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| 摘 要： | 本文报导了有机磷和氨基甲酸酯检测的一个新的酶抑制法.该法基于有机磷和氨基甲酸酯化合物对植物酶水解底物2,6-二氯乙酰靛酚的抑制作用.本法简单,快速,适于农药中毒的现场检验.其最小检出量分别为0.2～1μg/μ1和1～2μg/ml.
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| 关 键 词： | 植物酶 有机磷农药 氨基甲酸酯农药 试管法 薄层层析法 |
APPLICATION OF PLANT HYDRYOLASES IN THE DETERMINATION OF ORGANO-PHOSPHORUS AND CARBAMATE PESTICIDES IN POST-MORTEN SAMPLES |
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| Liu Jiuhua, Wang Qingfu, ShiKun Bureau of public security of Hebei Province,Shijiazhuang.APPLICATION OF PLANT HYDRYOLASES IN THE DETERMINATION OF ORGANO-PHOSPHORUS AND CARBAMATE PESTICIDES IN POST-MORTEN SAMPLES[J].Chinese Journal of Forensic Medicine,1989(2). |
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| Authors: | Liu Jiuhua Wang Qingfu ShiKun Bureau of public security of Hebei Province Shijiazhuang |
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| Abstract: | A new enzyme inhibition method for determination of organophosphorus and carbamate acompounds in the post-mortem samples has been developed. The principle of this method is based on the inhibiting action of organophosphorus and carbamate compounds to plant hydrolases which catalyticaily hydrolyze the substrate 2, 6-dich-lorophenolindophenol acetates. The method is simple, rapid and suitable for ensic examination of post-mortem samples for the pesticides poisoning. The minimum detecting amount of organophosphorus and carbamate compounds is 0.2～Img/ml and 1～2mg/ml respectivily. |
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| Keywords: | Plant hydrolases Organophosphorus pesticides Carbamate pesticides Test tube method TLC |
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