| 实验性脑挫伤后caspase-3表达的免疫组织化学研究 |
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| 引用本文： | 陈溪萍,陶陆阳,高玉伟,丁梅,官大威.实验性脑挫伤后caspase-3表达的免疫组织化学研究[J].中国法医学杂志,2003,18(2):74-77. |
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| 作者姓名： | 陈溪萍 陶陆阳 高玉伟 丁梅 官大威 |
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| 作者单位： | 1. 中国医科大学法医学院,辽宁,沈阳,110001
2. 沈阳市伤害法医鉴定所,辽宁,沈阳,110013 |
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| 摘 要： | 目的 观察大鼠在不同程度及不同时间脑挫伤后caspase-3表达的变化。方法 对48例实验性脑挫伤大鼠的脑组织进行检验,同时以10例非脑挫伤的脑组织做对照。结果 伤后1h,caspase-3即有表达;24～48h达高峰,2周时仍有阳性细胞存在。阳性细胞多集中于挫伤区及周围带皮质和脑实质内海马区;不同程度脑挫伤组之间于伤后1h,12h,24h,72h,1周和2周caspase-3表达有差异。结论 不同程度脑损伤后caspase-3表达量不同;在脑损伤后caspase-3的表达有时间变化规律,对探讨脑挫伤后二次损害原因和研究脑挫伤程度有一定意义。
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| 关 键 词： | 法医病理学 脑挫伤 caspase-3 免疫组织化学 凋亡 |
| 文章编号： | 1001-5728(2003)02-0074-04 |
| 修稿时间： | 2002年3月1日 |
A preliminary study on expression of caspase-3 in experimental brain contusion in rats |
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| CHEN Xiping,TAO Luyang,DING Mei,et al..A preliminary study on expression of caspase-3 in experimental brain contusion in rats[J].Chinese Journal of Forensic Medicine,2003,18(2):74-77. |
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| Authors: | CHEN Xiping TAO Luyang DING Mei |
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| Institution: | CHEN Xiping,TAO Luyang,DING Mei,et al. China Medical University,School of Forensic Medicine,Shenyang 110001 |
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| Abstract: | Objective To investigate the expression of caspase-3 in different post-traumatic intervals and severity of traumatic brain injury (TBI). Method Brain tissue samples of slight (n =24), moderate (n = 24) brain injury and control (n = 10) groups of rat were examined by immunohistochemical staining. Results Up-regulation of caspase-3 expression was found in tissues from traumatic brain injury as compared with controls in 1h after TBI, and lasted for 2 weeks. In tissues taken Ih, 12h, 24h, 72h, lweek and 2weeks after TBI there were different amount of positive celis in brain injuries of different severity. Con-clusion The expression of caspase-3 is different in brain injuries of different severity . These findings are valuable in appraising the severity of injuries. |
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| Keywords: | Forensic pathology Cerebral contusion Casapse-3 Immunohistochemistry Apoptosis |
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