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Assessing a novel room temperature DNA storage medium for forensic biological samples |
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| Authors: | Steven B Lee Kimberly C ClabaughBrie Silva Kingsley O OdigieMichael D Coble Odile LoreilleMelissa Scheible Ron M FourneyJesse Stevens George R CarmodyThomas J Parsons Arijana PozderArthur J Eisenberg Bruce BudowleTaha Ahmad Russell W MillerCecelia A Crouse |
| Institution: | a Forensic Science Program, Justice Studies Department, MacQuarrie Hall 521, San Jose State University, San Jose, CA 95192, United States b Research Section, Armed Forces DNA Identification Laboratory, Armed Forces Institute of Pathology, 1413 Research Blvd., Bldg 101, Rockville, MD 20850, United States c Biology Research and Development, Central Forensic Laboratory, Royal Canadian Mounted Police, Ottawa, ON, Canada K1G 3M8 d Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada K1S 5B6 e Forensic Sciences Division, International Commission on Missing Persons, Alipašina 45a, 71000 Sarajevo, Bosnia and Herzegovina f Forensic and Investigative Genetics and Institute of Investigative Genetics, University of North Texas Health Science Center at Fort Worth 3500 Camp Bowie Blvd., Fort Worth, TX 76107, United States g Marshall University, One John Marshall Drive, Huntington, WV 25755, United States h Palm Beach County Sheriff's Office, 3228 Gun Club Road, West Palm Beach, FL 33406, United States |
| Abstract: | The ability to properly collect, analyze and preserve biological stains is important to preserving the integrity of forensic evidence. Stabilization of intact biological evidence in cells and the DNA extracts from them is particularly important since testing is generally not performed immediately following collection. Furthermore, retesting of stored DNA samples may be needed in casework for replicate testing, confirmation of results, and to accommodate future testing with new technologies.A novel room temperature DNA storage medium, SampleMatrix™ (SM; Biomatrica, Inc., San Diego, CA), was evaluated for stabilizing and protecting samples. Human genomic DNA samples at varying amounts (0.0625-200 ng) were stored dry in SM for 1 day to 1 year under varying conditions that included a typical ambient laboratory environment and also through successive freeze-thaw cycles (3 cycles). In addition, spiking of 1-4× SM into samples prior to analysis was performed to determine any inhibitory effects of SM. Quantification of recovered DNA following storage was determined by quantitative PCR or by agarose gel electrophoresis, and evaluation of quantitative peak height results from multiplex short tandem repeat (STR) analyses were performed to assess the efficacy of SM for preserving DNA.Results indicate no substantial differences between the quality of samples stored frozen in liquid and those samples maintained dry at ambient temperatures protected in SM. For long-term storage and the storage of low concentration samples, SM provided a significant advantage over freezer storage through higher DNA recovery. No detectable inhibition of amplification was observed at the recommended SM concentration and complete profiles were obtained from genomic DNA samples even in the presence of higher than recommended concentrations of the SM storage medium. The ability to stabilize and protect DNA from degradation at ambient temperatures for extended time periods could have tremendous impact in simplifying and improving sample storage conditions and requirements. The current work focuses on forensics analysis; however this technology is applicable to all endeavors requiring storage of DNA. |
| Keywords: | DNA storage DNA recovery Short tandem repeat analysis Freeze-thaw Trehalose SampleMatrix QIAsafe DNA stability |
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