| 酶标2G8单克隆抗体斑点ELISA快速检验人血痕G2m(23)因子 |
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| 引用本文： | 蒯应松,王香菊,徐秀兰,杨元立,吴贤柱,崔家贵,李万水,谢英群.酶标2G8单克隆抗体斑点ELISA快速检验人血痕G2m(23)因子[J].中国法医学杂志,1993(4). |
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| 作者姓名： | 蒯应松 王香菊 徐秀兰 杨元立 吴贤柱 崔家贵 李万水 谢英群 |
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| 作者单位： | 公安部第二研究所 (北京100038) |
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| 摘 要： | 利用自制的抗人 G2m(23)酶标2G8酶标单克隆抗体,首次应用直接斑点 ELISA 方法检测血痕中 G2m(23)因子。该法最小检出量为0.000048μl 血清;整个试验可在15分钟内完成。对常见的13种动物血和8种鱼血无交叉反应;盲测450例干血痕结果全部正确。该方法具有快速、准确、灵敏、简便等特点,有较大的实用价值。
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| 关 键 词： | 单克隆抗体 G2m(23)因子 斑点ELISA |
APPLICATION WITH ENZYME LABELED MONOCLONAL ANTIBODY AGAINST G2m(23)ANTIGEN FOR RAPID DETECTION OF G2m(23)FACTOR IN BLOODSTAIN BY DOT-ELISA |
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| Kuai Yingsong Institute of Forensic Science Ministry of Public Security,Beijing.APPLICATION WITH ENZYME LABELED MONOCLONAL ANTIBODY AGAINST G2m(23)ANTIGEN FOR RAPID DETECTION OF G2m(23)FACTOR IN BLOODSTAIN BY DOT-ELISA[J].Chinese Journal of Forensic Medicine,1993(4). |
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| Authors: | Kuai Yingsong Institute of Forensic Science Ministry of Public Security Beijing |
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| Institution: | Kuai Yingsong Institute of Forensic Science Ministry of Public Security,Beijing,100038 |
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| Abstract: | The direct dot-ELISA method used for detecting G2m(23)factors in human serum and blood-stain was established by using monoclonal antibodies 2G8 raised against G2m(23)antigen.There Wasno cross reaction with bloodstains from13 animals.G2m(23)factors in 450 human bloodstains weresurveyed with 100% accuracy.The method was rapid,accurate sensitive and easy to perform.As lit-tle as 0.000048μl blood can he tested correctly in 15mini.So it can be used in forensic science labora-tory. |
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| Keywords: | Monoelonal antibody G2m(23)Dot-ELISA |
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