| PCR－RFLP技术鉴定ABO基因型的法医学应用研究 |
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| 作者姓名： | Wang XL Cheng XZ Liu C Liu C Yang W Zhang JF |
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| 作者单位： | 王孝力（中山市刑事科学技术研究所,广东 528403）;程新志（中山市刑事科学技术研究所,广东 528403）;梁敏捷（中山市刑事科学技术研究所,广东 528403）;刘超（广州市刑事科学技术研究所,广东 510060）;杨巍（中山市刑事科学技术研究所,广东 528403）;张继富（中山市刑事科学技术研究所,广东 528403） |
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| 摘 要： | 目的建立PCR－RFLP、非变性PAG胶垂直电泳和银染技术进行ABO基因分型的方法体系,并对200名广东汉族人群ABO基因型频率进行了调查。方法用Chelex－100和酚、氯仿抽提法处理样本,PCR扩增后用非变性聚丙烯酰胺凝胶垂直电泳和银染技术检测分型。结果ABO位点特异性扩增片段长度为175bp～210bp,6种基因型频率分布为0.0250～0.4300,杂合度H值为0.5162,个体识别力DP值为0.7111。结论该方法可成功运用于血液、血痕、精斑、毛发、骨组织和混合斑等检材的个体识别及亲权鉴定的检验。
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| 关 键 词： | ABO基因分型 聚合酶链式反应（ PCR） 限制性片段长度多态性（ RFLP） |
| 文章编号： | 1004－ 5619(2001)03－ 0152－ 03 |
| 修稿时间： | 2000年6月14日 |
Study on the forensic application of ABO genotyping by PCR-RFLP |
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| Wang XL,Cheng XZ,Liu C,Liu C,Yang W,Zhang JF.Study on the forensic application of ABO genotyping by PCR-RFLP[J].Journal of Forensic Medicine,2001,17(3):152-154. |
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| Authors: | Wang X L Cheng X Z Liu C Liu C Yang W Zhang J F |
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| Institution: | Forensic Science Institute of Zhongshan City, Zhongshan 528403. |
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| Abstract: | OBJECTIVE: The study was carried out on the application of ABO genotyping by PCR-RFLP to forensic purpose. METHODS: The DNA of samples were extracted by Chelex--100 and phenol/chloroform, the PCR products were separated by Native Horizontal PAGE, and detected by silver staining. RESULTS: The PCR amplified fragment length of ABO loci is from 175 to 210 bp, the frequency distribution of six genotypes ranges from 0.0250 to 0.4300, heterozygosity(H) and discriminating power(Dp) is 0.5162 and 0.7111. CONCLUSION: This technique has a good application to the forensic samples such as blood, blood stain, semen stain, hair root, bone tissue and semen from vaginal swabs. |
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