VEGF在早期心肌梗死死后诊断中的应用

运用免疫组化方法和图像分析与统计学处理系统 ,对人体心脏标本VEGF的表达进行定量研究。结果显示 :心肌梗死组 2 4例心脏标本心肌细胞膜及血管内皮细胞膜均可见棕黄色VEGF强阳性染色 ,有些胞浆也呈阳性 ,尤以心肌梗死灶周围区域心肌细胞为甚 ;在可疑梗死组 2 1例中 ,19例心肌细胞膜及血管内皮细胞膜VEGF强阳性 ,2例为弱阳性 ,未见阴性 ;在正常心脏对照组 16例中 ,仅 1例见心肌细胞膜及血管内皮细胞膜散在棕黄色VEGF弱阳性 ,其余均为阴性。图像分析与统计学处理结果表明 ,对照组VEGF阳性指数 ( 0 3 0± 0 10 )明显低于心肌梗死组 ( 12 46± 3 2 6,P<0 0 1)和可疑梗死组 ( 11 70± 3 5 6,P <0 0 1) ,后两组之间阳性指数无明显差异 (P >0 0 5 )。提示运用免疫组化染色方法结合图像分析处理技术定量检测心肌局部VEGF的表达可望作为因早期心肌缺血导致心脏性猝死死后诊断较为客观的病理形态学指标之一。

The immunohistochemical study of VEGF for postmortem diagnosis of early myocardial infarction

With the use of immuno histochemical SABC VEGF staining and Image Quantification Analysis as well as Statistics Process System,the expression of vascular endothelial growth factor (VEGF) was firstly studied in 61hearts from autopsy for the purpose of postmortem diagnosis of early myocardial infarction The results revealed that all 24 cases of confirmed infarcts had positive staining of VEGF on membranes of myocardial cell and vascular endothelia. Positive expression of VEGF in cytoplasm of myocardial cells were found in some cases,especially at the border zone of infarction Among 21 cases with suspect caridac infarction,VEGF was strongly positive in 19 cases and weak positive in 2 cases,whereas only mild positive staining of VEGF in myocardial cells and in vascular endothelium in one case of normal conerol group.The rest cases were negative Positive indexc (Pl) of normal hearts was 0 30±0 10 which was significantly lower than that of confirmed infarcts and suspect infarcts(12 46±3 26 and 11 70±3 56 respectively, P <0 01).No significant difference between PI of infarcts and of suspect infarcts was found( P >0 05) This finding suggests that SABC VEGF method provides an objective morphologic evidence for the postmortem diagnosis of sudden cardiac death caused by early myocardial infarction