大鼠局灶性脑挫伤后Homer蛋白表达变化

目的 观察局灶性脑挫伤后Homer蛋白表达的变化规律,探讨其表达变化与损伤时间的相关性.方法 建立大鼠钝力性局灶性脑挫伤模型,应用免疫组织化学技术和Western印迹法观察脑损伤后不同时间脑组织中Homer蛋白表达的变化.结果 对照组、假手术组及伤后0.5h组脑组织中有少量Homer蛋白染色阳性细胞,伤后3h组脑组织H...

Homer protein expression after focal brain contusion in rats

Objective To investigate Homer protein expression after focal brain contusion and explore the relationship between expression and injury time.Methods Focal brain contusion in rats was established and Homer protein expression in brain at different injury intervals after contusion was detected by immunohistochemistry and Western blotting.Results A small amount of Homer positive expression cells were detected in control group,sham operated group and experimental group（0.5 h after contusion）.The amount of Homer positive expression cells increased after 3 h and reached peak 12 h after contusion.The amount of positive cells continued to decrease 1 d after contusion and to the base level 7 d after contusion.Homer protein expression based on immunohistochemistry and Western blotting had statistical difference among adjacent groups.Conclusion Expression of Homer protein near the focal contusion area shows time dependence after brain contusion in rats.