小鼠脑外伤后Apelin-13对自噬相关蛋白表达的影响 |
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引用本文: | 蒯锦霞,包海军,王英元.小鼠脑外伤后Apelin-13对自噬相关蛋白表达的影响[J].中国法医学杂志,2014(4):322-325. |
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作者姓名: | 蒯锦霞 包海军 王英元 |
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作者单位: | 山西医科大学法医学院;徐州医学院病理教研室; |
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摘 要: | 目的观察Apelin-13对小鼠脑外伤(Traumaticbraininjury,TBI)自噬相关蛋白的影响,并初步探讨其对小鼠脑外伤后自噬相关蛋白调节的作用机制。方法取健康雄性CD-1小鼠,随机分为假手术(sham)组、生理盐水(sa-line)组、Apelin-13组。Apelin-13组和saline组在建立脑外伤模型前10min,分别注射Apelin-13(0.3μg/g)或等量生理盐水于侧脑室,然后利用Western-Blot检测脑外伤后24h、48h组大脑皮质自噬相关蛋白LC3、P62、Beclin-1和Bcl-2等的表达情况。结果与sham组相比,在脑外伤后24h、48h,saline组自噬相关蛋白LC3-Ⅱ/LC3-Ⅰ比值,Beclin-1的蛋白表达均被显著上调(P〈0.01);同时Bcl-2、P62的蛋白表达水平被显著下调。然而,与saline组相比,Apelin-13组可显著下调自噬相关蛋白LC3-Ⅱ/LC3-Ⅰ比值和Beclin-1的蛋白表达水平(P〈0.01);同时显著上调Bcl-2和P62的表达水平;亦明显降低Beclin-1/Bcl-2的比值。结论Apelin-13可以明显抑制脑外伤后小鼠大脑皮质细胞的自噬活性,这一作用可能是通过调节Beclin-1、Bcl-2蛋白表达水平来实现的。
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关 键 词: | 法医病理学 Apelin-13 脑外伤 自噬 Beclin-1 |
The effect of Apelin-13 on autophagy-associated protein expression in mouse cerebral cortex after traumatic brain injury |
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Institution: | Kuai Jinxia, Bao Haijun, Wang Yingyuan(1. Shan Xi Medicat University School of Forensic Medicine, Taiyuan, 030001, China; 2. Department of Pathology, Xu Zhou Medical Col- lege, Jiangsu Xuzhou, 221000, China) |
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Abstract: | Objective To observe the effect of apelin-13 on traumatic brain injury (TBI), and explore the relationship between apelin-13 and autophagy in TBI. Methods 30 mice were randomly divided into sham, saline and apelin-13 groups. Apelin-13 and saline groups were pretreated with an injection of apelin- 13(0.3μg/g) and saline into the mouse brain lateral ventricle respectively 10 min before TBI. The autoph- agy-associated proteins LC3, P62, Beclin-1 and Bcl-2 in the mouse cerebral cortex were assessed by Western-Blot 24h and 48h after TBI. Results Compared with sham group, protein LC3-Ⅱ/LC3-Ⅰ ratio and Beclin-1 expression were up-regulated, while protein Bel-2 and P62 were down-regulated in saline group 24h or 48h after TBI. However, compared with saline group, Apelin-13 decreased protein LC3-Ⅱ/LC3-Ⅰratio and Beclin-1 expression, increased protein Bcl-2 and P62 expression ,and down-regulated protein Beclin-1/ Bcl-2 ratio 24h or 48h after TBI. Conclusion Apelin-13 suppresses autophagy in TBI possibly by down-regulating protein Beclin-1/Bcl-2 ratio. |
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Keywords: | forensic pathology Apelin-13 traumatic brain injury (TBI) autophagy Beclin-1 |
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