| 生物检材中阿维菌素的HPLC—MS／MS分析 |
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| 引用本文： | 董颖,王炯,何毅.生物检材中阿维菌素的HPLC—MS／MS分析[J].中国法医学杂志,2009,24(2):96-98. |
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| 作者姓名： | 董颖 王炯 何毅 |
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| 作者单位： | 公安部物证鉴定中心,北京,100038 |
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| 摘 要： | 目的建立生物检材包括血、肝组织、胃组织中阿维菌素（Avermectins）的HPLC—MS／MS分析方法。方法采用Oasis HLB固相萃取柱进行提取，以XTerra^TM RP18柱（2．1mm×100mm，3．5μm）色谱柱分离，以甲醇-0．1％冰醋酸水溶液（75：25）为流动相，流速为0．2mL／min。结果线性范围10ng／mL～3μg／mL，最小检出限为0．1ng／mL。结论本方法准确、快速，可用于生物检材血中阿维菌素的定性定量分析，肝及胃组织中阿维菌素的定性分析。
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| 关 键 词： | 法医毒物分析 阿维菌素 生物检材 固相萃取 HPLC-MS/MS |
Determination of avermectins in biological human samples by HPLC-MS/MS |
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| DONG Ying,WANG Jiong,HE Yi.Determination of avermectins in biological human samples by HPLC-MS/MS[J].Chinese Journal of Forensic Medicine,2009,24(2):96-98. |
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| Authors: | DONG Ying WANG Jiong HE Yi |
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| Institution: | DONG Ying,WANG Jiong,HE Yi/ Institute of Forensic Science Ministry of Public Security,Beijing 100038,China |
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| Abstract: | Objective To establish HPLC-MS/MS method for the determination of avermectins in biological Human Samples. Methods Samples were extracted by method of solid-phase extraction. XTerraTM RP18 column (2.1 mm×100 mm, 3.5 μm) and the mobile phase with methanol:acetic acid of 0.1% (75∶25) were used. The flow rate was 0.2 mL·min-1. Results There was a good linear relationship within the range of 10 ng/mL～3 μg/mL. The limit of detection was 0.1ng/mL . Conclusions The method is simple, rapid, accurate . It can be used for the determination of avermectins in biological human samples. |
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| Keywords: | HPLC-MS/MS |
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