用ELISA和PCR检测异常妊娠妇女弓形虫感染

为了解兰州市妊娠妇女近年弓形虫感染情况 ,探讨异常妊娠与弓形虫感染的关系 ,分别采用ELISA和PCR检测了异常妊娠妇女血清中TOX IgM和TOX DNA。结果 ,1 3 5例异常妊娠妇女血清TOX IgM和TOX DNA阳性率分别为 1 1 .1 1 % (1 5 /1 3 5 )和 1 2 .5 9% (1 7/1 3 5 ) ,总阳性率为 1 3 .3 3 % (1 8/1 3 5 ) ,组间无显著差异 (P >0 .0 5 )。异常妊娠史妇女与妊娠期无异常孕妇的血清TOX IgM阳性率分别为 1 1 .1 1 % (1 5 /1 3 5 )和 6.0 2 % (3 2 /5 3 2 ) ,组间差异显著 (P <0 .0 5 ) ,相对危险度为1 .85。调查结果显示 ,兰州市弓形虫血清抗体阳性率较 1 994年略有上升 ,弓形虫感染的孕前检查应联合应用ELISA和PCR为宜。

Detection of Toxoplasma gondii infection in abnormal pregnant women by the ELISA and PCR

In order to understand Toxoplasma gondii infection in pregnant women in Lanzhou region in Gansu province of China, and to study the relationship between the infection and abnormal pregnant, Toxoplasma gondii IgM or DNA in sera of abnormal pregnant women were detected by ELISA or PCR . The results showed that IgM and DNA positive percentages were 11.11% (15/135) and 12.59%(17/135) in sera of 135 abnormal pregnant women respectively, and the difference between them was no significant (P>0.05). It was also showed in the results that IgM positive percentages were 11.11% (15/135) and 6.02% (32/532) in the abnormal pregnant women and the normal pregnant ones respectively, and the difference between them was significant (P<0.05). It is concluded that Toxoplasma gondii IgM positive percentage has risen in Lanzhou, and ELISA and PCR together can be used to diagnosing toxoplasmosis in pregnant women.