共振光散射法在核酸测定中的应用研究 |
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引用本文: | 夏忠瑜.共振光散射法在核酸测定中的应用研究[J].四川警官高等专科学校学报,2011(6):106-111. |
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作者姓名: | 夏忠瑜 |
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作者单位: | 四川警察学院,四川泸州646000 |
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摘 要: | 本文用RLS技术研究了在酸性介质中,阳离子表面活性剂溴化十六烷基三甲铵(CTMAB)存在下,依来铬蓝黑R(Eriochrome Blue Black R,EBBR)与鱼精脱氧核糖核酸(fsDNA)相互作用的RLS光谱特征及影响因素,并在优化实验条件下,实现了对fsDNA的定量检测。对于EBBR┝CTMAB—fsDNA体系,fsDNA浓度的线性范围为0.01-1.6μg/mL,相应的检出限(3σ,n=5)为4.6ng/mL。将所建立的方法用于合成样品中痕量核酸的测定,回收率在97%-106%之间,RSD小于2.8%。
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关 键 词: | 共振光散射 依来铬蓝黑R 溴化十六烷基三甲铵 核酸测定 |
Studies on the Application of Resonance Light Scattering Technique in Nucleic Acids Determination |
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Authors: | XIA Zhong-yu |
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Institution: | XIA Zhong-yu |
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Abstract: | In acid medium, the interaction between fsDNA and Eriochrome Blue Black R (EBBR) in pretence of cetyltrimethylammonium bromide (CTMAB) Was studies using RLS method respectively. RLS spectra of the system Was measured and the influence factors were investigated. Under optimized condition, there is a linear relationship between the concentration of fsDNA and RLS intensity of the system (EBBR- CTMAB -fsDNA) in the ranges from 0.01 to 1.6 μg/mL, with detection limit of 4.6 ng/mL (3σ,n = 5), respectively. This new approach was applied to determine trace nucleic acids in synthetical sample, the recovery vercent was 97% to 106% and RSD was less than 2.8%. |
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Keywords: | Resonance Light Scattering Eriochrome Blue Black R Cetyltrimethylammonium Bromide Determination of Nucleic Acids |
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