| 猪生殖与呼吸综合征病毒GP5基因对小鼠的DNA免疫 |
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| 引用本文： | 任慧英,杨汉春,郭鑫,陈艳红,查振林.猪生殖与呼吸综合征病毒GP5基因对小鼠的DNA免疫[J].中国兽医科学,2006,36(2):107-111. |
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| 作者姓名： | 任慧英 杨汉春 郭鑫 陈艳红 查振林 |
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| 作者单位： | 中国农业大学,农业部预防兽医学重点实验室,北京,100094 |
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| 基金项目： | 国家科技攻关项目;科技部专项基金 |
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| 摘 要： | 将表达完整猪生殖与呼吸综合征病毒(PRRSV)GP5和融合表达泛素与GP5成熟肽的真核重组表达质粒(pCI-GP5和pcDNA-U-GP5)分别肌肉注射免疫BALB/c小鼠,共免疫3次。采用间接ELISA法检测血清中抗GP5的抗体水平,MTT法检测脾淋巴细胞的增殖能力,LDH释放试验检测脾NK细胞的杀伤活性,并通过流式细胞术检测脾淋巴细胞亚群的比例。结果发现,2种DNA重组质粒免疫后均可诱导小鼠产生体液免疫和细胞免疫。三免后第5周,pCI-GP5质粒免疫组诱导产生的抗GP5抗体水平及NK活性明显高于pcDNA-U-GP5质粒免疫组;而pcDNA-U-GP5免疫组的淋巴细胞增殖指数及CD4-CD8 T细胞比例明显高于pCI-GP5组。表明,GP5基因可诱导产生较高的免疫反应,泛素与GP5的融合表达可增强PRRSV GP5诱导的细胞免疫反应。
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| 关 键 词： | 猪生殖与呼吸综合征病毒 泛素 DNA免疫 小鼠 |
| 文章编号： | 1673-4696(2006)02-0107-05 |
| 修稿时间： | 2005年9月21日 |
Immunization of mice with recombinant DNA vaccines encoding PRRSV GP5 gene |
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| REN Hui-ying,YANG Han-chun,GUO Xin,CHEN Yan-hong,ZHA Zhen-lin.Immunization of mice with recombinant DNA vaccines encoding PRRSV GP5 gene[J].Veterinary Science in China,2006,36(2):107-111. |
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| Authors: | REN Hui-ying YANG Han-chun GUO Xin CHEN Yan-hong ZHA Zhen-lin |
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| Abstract: | BALB/c mice were vaccinated intramuscularly with DNA vaccine vectors pCI-GP5 and(pcDNA)-U-GP5 which contain porcine reproductive and respiratory syndrome virus(PRRSV) GP5 gene with or without porcine ubiquitin gene.On week 5 after the third immunization,mice in pCI-GP5 group showed much higher antibody levels and percentage of NK lysis than that in pcDNA-U-GP5 group.A significantly enhanced stimulation index and a significantly increased percentage of CD4~(-)CD8~( )T cells were observed in pcDNA-U-GP5 group compared with that in pCI-GP5 group.The results demonstrated that DNA immunization against PRRSV induced the production of both humoral-and cell-mediated immune(responses) in mice.The pcDNA-U-GP5 could elicit much stronger cellular immune response. |
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| Keywords: | GP5 |
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