Application of species-specific polymerase chain reaction in the forensic identification of tiger species |
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Authors: | Wan Qiu-Hong Fang Sheng-Guo |
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Institution: | Key Laboratory of Conservation Genetics and Reproductive Biology for Endangered Wild Animals, Ministry of Education, Zhejiang, PR China. |
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Abstract: | Globally, tigers are considered to be endangered, and are listed on Appendix I of CITES. A simple test, using a species-specific primer pair, was developed to identify tiger meat, faeces and dried skin, and provide forensic evidence of illegal wildlife trade. The specific fragment of mitochondrial cytochrome b gene was also successfully amplified from raw DNA products extracted from single tiger hairs. This PCR-based approach opens a new avenue to forensic identification of less-than-optimal samples. |
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