利用PCR检测鸭瘟病毒

参照文献 ,设计和合成了 1对引物。用这对引物 ,以标准毒株DPVF3 4的DNA为模板 ,经PCR扩增出 1个特异的DNA片段。经序列测定 ,该DNA片段由 42 0bp组成。与文献报道的序列比较 ,该片段为鸭瘟病毒UL6基因DNA的一部分 ,与美国毒株LakeAndes(LA)的同源性达 99%。用该PCR方法扩增小鹅瘟病毒、细小病毒和禽巴氏杆菌 ,结果为阴性。以该PCR方法检测 6份送检病料 ,5份为阳性 ,检出率与病毒的分离一致。另外 ,该方法检测DPVDNA的敏感性达到了 1pg水平

Detection of duck plague virus by PCR

A UL6 gene fragment of duck plague virus (DPV) was amplified with PCR and sequenced. The results indicated that this DNA fragment was composed of 420 bp nucleotides. Comparison of the nucleotide sequences and deduced amino acid sequences of the UL6 gene fragment with that of other DPV strians revealed that there were only one nucleotide change with 99% homology and the deduced amino acid sequences were same. The sensitivity and specificity of the PCR method detecting DPV with PCR were tested. The PCR can yield 420 bp special DNA band only from DPV and minimum DNA model was 1.0 pg. Six samples from dead ducks were diagnosed by PCR, and five of them were positive. The result was the same as one of virus isolation method.