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| 人血浆中更昔洛韦的高效液相色谱快速分析 | |
| 引用本文： | 金鸣,李强锋,黄飞,高丽伟,刘缙.人血浆中更昔洛韦的高效液相色谱快速分析[J].中国法医学杂志,2010,25(1). |
| 作者姓名： | 金鸣 李强锋 黄飞 高丽伟 刘缙 |
| 作者单位： | 1. 华中科技大学同济医学院法医学系,湖北,武汉,430030 2. 南安市公安局刑侦大队,福建,南安,362200 3. 三峡大学医学院,湖北,宜昌,443000 |
| 摘 要： | 目的建立人血浆中抗病毒药物更昔洛韦的高效液相色谱快速分析方法。方法在一定pH值下用甲醇和二氯甲烷处理血浆,高速离心后外标法直接对上清液进行更昔洛韦定性、定量分析。以正常人血浆为空白样本添加标准更昔洛韦,优化色谱分析条件并确定检材的前处理方法、工作曲线、线性范围、方法的精密度和回收率。以所建方法对12位受试者血浆中更昔洛韦代谢浓度进行12h监测。结果该方法分析血浆中的更昔洛韦,其线性范围为0.05μg/mL～1.60μg/mL;最低检测限为0.01μg/mL(S/N≥3),日内、日间RSD≤5.1%,加样回收率在90.0%～95.4%之间。结论该分析方法操作便捷、实用、准确度高。适用于更昔洛韦的临床血药浓度监测和法医学药(毒)物快速鉴定。 |
| 关 键 词： | 法医毒物分析 更昔洛韦 血浆 高效液相色谱 |
Determination of ganciclover in human plasma by reversed-phase high performance liquid chromatography |
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| JIN Ming,LI Qiangfeng,HUANG Fei,GAO Liwei,LIU Jin.Determination of ganciclover in human plasma by reversed-phase high performance liquid chromatography[J].Chinese Journal of Forensic Medicine,2010,25(1). | |
| Authors: | JIN Ming LI Qiangfeng HUANG Fei GAO Liwei LIU Jin |
| Institution: | JIN Ming1;LI Qiangfeng2;HUANG Fei3;GAO Liwei1;LIU Jin1 /1.Faculty of Forensic Medicine;Tongji Medical College;Huazhong Science and Technology University;Wuhan;430030;2.Public Security Bureau of NanAn county;Fujian Nanan 362200 China;3.Medical Science College of China Three Gorges University;Hubei Yichang;443000;China |
| Abstract: | Objective To develop a method for the determination of ganciclover in human plasma by RP-HPLC.Methods Plasma containing ganciclover was extracted with methanol and methylene chloride,qualitative and quantitative analysis was carried out directly.Working curve,linear range,recovery,precision and so on was obtained according to the sample pre-processing method and analysis state.The HPLC method has been taken to investigate the plasma concentration of ganciclover for 12 volunteers.Results The relationship of ... |
| Keywords: | forensic toxicology analysis ganciclover plasma HPLC |
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