| 线粒体16SrRNA和Cytb基因复合扩增进行种属鉴定 |
| |
| 作者姓名： | Ye Y Wu J Luo HB Wang Z Li YB |
| |
| 作者单位： | [1]四川大学华西基础医学与法医学院物证教研室,四川成都610041 |
| |
| 摘 要： | 目的 建立一种用于种属鉴定的线粒体DNA16SrRNA基因和细胞色素b基因荧光标记复合扩增检测体系。方法 利用引物设计软件Primer 5．0对mtDNA序列的16SrRNA基因和细胞色素b基因各设计一对引物,建立复合扩增体系,分别扩增人和牛、猪、狗、鸡、草鱼5种常见动物,用310遗传分析仪对产物进行分析。结果 人和5种动物DNA扩增产物均出现两个峰。Cytb通用引物的扩增产物为人与动物的共有峰,为358bp;16SrRNA基因的扩增产物为人与动物间存在位置差异的特异峰,位于231～256bp之间。结论 该复合扩增体系可以明确区分人和5种动物样本,可用于种属鉴定。
|
| 关 键 词： | 法医遗传学 生物学鉴定法 基因扩增 DNA 线粒体 基因 16SrRNA 基因 细胞色素B |
Multiple amplification of 16S rRNA gene and Cytb gene in mitochondrial DNA for species identification |
| |
| Ye Y,Wu J,Luo HB,Wang Z,Li YB.Multiple amplification of 16S rRNA gene and Cytb gene in mitochondrial DNA for species identification[J].Journal of Forensic Medicine,2008,24(4):259-261. |
| |
| Authors: | Ye Yi Wu Jin Luo Hai-bo Wang Zhuo Li Ying-bi |
| |
| Institution: | Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China. yeyiconan@sina.com |
| |
| Abstract: | OBJECTIVE: To establish a fluorescent multiple amplification system of 16SrRNA and Cytb genes located in mitochondrial DNA for species identification. METHODS: A pair of primers of 16SrRNA gene and Cytb gene of the mitochondrial DNA was designed with the software Primer 5.0 to construct a multiple amplification system. The amplified products from human and five species of animals, including cattle, pig, dog, chicken and grass carp were analyzed by 310 Genetic Analyzer. RESULTS: The amplified products of these samples showed two peaks. The common one was 358bp and the specific one different in unique species was between 231bp and 256bp. CONCLUSION: The multiplex amplification system can exactly distinguish the species of human from five common animals. |
| |
| Keywords: | forensic genetics biological assay gene amplification DNA mitochordria genes 16SrRNA genes cytochromes b |
| 本文献已被 维普 PubMed 等数据库收录！ |
