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| 液相色谱质谱联用测定乌头碱在大鼠体内代谢产物 | |
| 引用本文： | 王朝虹,文静,陈义华,何毅.液相色谱质谱联用测定乌头碱在大鼠体内代谢产物[J].中国法医学杂志,2006,21(2):88-90. |
| 作者姓名： | 王朝虹 文静 陈义华 何毅 |
| 作者单位： | 1. 公安部物证鉴定中心,北京,100038 2. 南昌铁路中心医院,江西,南昌,330000 3. 南昌市第三医院,江西,南昌,330000 |
| 摘 要： | 目的鉴定乌头碱在大鼠体内的主要代谢产物。方法灌胃给予雄性大鼠1.0mg/kg乌头碱后,收集24h尿液,固相萃取法提取,液相色谱-质谱法测定乌头碱及其代谢物。结果经与空白组对照发现给药后大鼠尿样中除乌头碱原体外还有4种代谢产物,并分别测得其准分子离子峰及其各级碎片离子。结论经与对照品比较及质谱断裂规律推断4个代谢产物分别为中乌头碱、16-O-去甲基乌头碱、16-O-去甲基中乌头碱、苯甲酰乌头碱。 |
| 关 键 词： | 法医毒物学 乌头碱 代谢物 液相色谱-质谱法 |
| 文章编号： | 1001-5728(2006)02-0088-03 |
| 修稿时间： | 2005年1月4日 |
Study on determination of metabolites of aconitine in rat urine by HPLC/MS |
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| Abstract: | Objective To determine the main metabolites of aconitine in urine in rats.Methods After oral administration of 1.0mg/kg aconitine,the urine of male rats was collected within 24h.The urine was extracted by solid phase extraction and analyzed by liquid chromatography –mass spectrometry.Results Aconitine and 4 metabolites were detected in the rat urine,and their protonate molecular ions and multistage fragment ions were monitored as compared with the blank control.Conclusion The metabolites were identified as mesaconitine,16-o-demethylaconitine,16-odemethylmesaconitine and benzoylaconitine,respectively. |
| Keywords: | Forensic toxicology Aconitine Metabolites HPLC/MS |
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