| 用反相杂交技术对HLA-DQA1基因分型及其应用 |
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| 作者姓名： | Lin Y |
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| 作者单位： | 1. 司法部司法鉴定科学技术研究所,上海,200063
2. 上海医科大学,上海,200032 |
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| 摘 要： | 目的 :HLA -DQA1其因的分型研究及在实际办案中的应用。方法 :PCR结合反相杂交检测技术对HLA -DQA1基因进行分型。结果 :检见7种基因 ,24种基因型 ,获得上海地区HLA -DQA1基因的基因频率及基因型的频率分布数据。结论 :对法医学中常见的血液 (痕 )、牙齿、精斑、混合斑、人体组织等检材进行HLA -DQA1分型检测 ,其结果稳定可靠 ,为实际办案提供了科学依据
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| 关 键 词： | PCR技术 反相杂交检测技术 HLA-DQA1基因 亲权鉴定 个体识别 |
| 修稿时间： | 1999－09－24) |
Validation studies on the analysis of the HLA-DQA1 locus using PCR and its application in forensic science |
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| Lin Y.Validation studies on the analysis of the HLA-DQA1 locus using PCR and its application in forensic science[J].Journal of Forensic Medicine,2000,16(1):12-13. |
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| Authors: | Lin Y |
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| Institution: | Institute of Forensic Science, Ministry of Justice, Shanghai 200063, P. R. China. |
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| Abstract: | Validation Studies were conducted to evaluate the forensic applicability of HLA-DQA1 locus. The locus was typed by a reverse dot blot approach and allele specific oligonucleotide probes were immobilized on a nylon membrane strip. Allele and genotype frequency for HLA-DQA1 locus was determined in shanghai Han population. The frequency data can be used in individual identification and paternity test. |
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| Keywords: | PCR Reverse Dot Blot HLA-DQA1 paternity test individual identification |
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