猪伪狂犬病病毒JSZ株的分离鉴定及其病毒核酸的分布规律

用猪伪狂犬病病毒(PRV)特异性引物对江苏省某猪场疑似猪伪狂犬病的病死仔猪组织病料进行了PCR鉴定,结果可扩增到特异性的条带.用该病料接种PK-15细胞,24 h即出现细胞病变,PCR和间接免疫荧光试验结果均证明所分离病毒为PRV.用该PRV分离株接种兔后出现奇痒症状并麻痹致死;接种仔猪后出现持续发热,肌肉震颤等症状.通过对自然感染PRV病死仔猪脏器进行PCR检测,发现PRV在仔猪体内广泛分布,其中以脾的检出率最高,可达100%;对人工感染猪的直肠和鼻腔棉拭样品进行PCR检测,发现病猪排毒最长可达13 d.证实,该分离株为猪伪狂犬病强毒株,脾为病毒检测的首选靶器官.

Isolation and identification of porcine pseudorabies virus strain JSZ and its nucleotide distribution in vivo

A specific band was amplified from suspicious samples collected from dead piglets on a Jiangsu pig farm using PCR with a pair of specific primers for porcine pseudorabies virus(PRV). The cyto-pathic effect was observed in PK-15 cells infected with the samples at hour 24 post-inoculation. The PRV infected PK-15 cells was identified by PCR method and indirect immunofluorescence assay. The PRV isolate resulted in intense itch and death in challenged rabbits,and in persistent fever and seizures in chal-lenged piglets. The PRV nucleotides were detected widely in organs and tissues of the naturally infected piglets by PCR method,and the detection ratio in spleen was as high as 100%. In experimental challenge, the period of PRV shedding from infected piglets was about 13 d when the swab samples collected from rec-tum or nasal cavity were detected by PCR. The results revealed that the PRV isolate was virulent and the spleen was the most sensitive target organ for PRV detection.