| 联合应用多个DNA位点进行尸源鉴定 |
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| 引用本文： | 杨电,刘超,王穗保.联合应用多个DNA位点进行尸源鉴定[J].法律与医学杂志,2000,7(4):149-152. |
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| 作者姓名： | 杨电 刘超 王穗保 |
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| 作者单位： | 广州市刑事科学技术研究所!510030 |
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| 基金项目： | 广州市重点科技攻关项目!(97-Z -8601)资助课题 |
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| 摘 要： | 目的 研究联合应用多个DNA位点在尸源鉴定方面的应用价值。方法 应用聚合酶链反应(PCR)、聚丙烯酰胺凝胶电泳分离及银染显带的方法通过对无名尸体的有关检材与可疑双亲或子女进行亲权鉴定。结果 在 80例刑事案件尸源鉴定中 ,38例无名尸体采用较新鲜肌肉 ,通过扩增VNTR、STR多个位点得以判明尸源 ;2 9例采用腐败肌肉、 6例采用骨骼和 2例采用牙齿 ,通过扩增多个STR位点判明了尸源。仅有 1例采用腐败肌肉、 2例采用骨骼未能确定尸源。结论 运用多个位点进行亲权方法可以准确地判明尸源鉴定 ,特别对高度腐败尸体、尸块不全等情况下的尸源鉴定具有很高的实用价值
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| 关 键 词： | 聚合酶链反应(PCR) DNA遗传多态性 可变数目串联重复(VNTR) 短串联复(STR) 尸源鉴定 |
| 文章编号： | 1007-9297（2000）04-0149-04 |
| 修稿时间： | 1999年11月25日 |
Corpses identification using STR and VNTR loci system |
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| YANG Dian,LIU Chao,WANG Sui-bao Forensic Science Institute,Guangzhou Public Security Bureau.Corpses identification using STR and VNTR loci system[J].The Journal of Law & Medicine,2000,7(4):149-152. |
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| Authors: | YANG Dian LIU Chao WANG Sui-bao Forensic Science Institute Guangzhou Public Security Bureau |
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| Institution: | YANG Dian,LIU Chao,WANG Sui-bao Forensic Science Institute,Guangzhou Public Security Bureau 510030 |
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| Abstract: | Objective To explore the practice value of corpses identification using STR and VNTR loci system Methods The tissue samples collecting from unknown corpses were carried out by test Paternity with probable parents or children using PCR, PAGE and silver stains Result In 80 criminal cases, the sample of fresher muscle in 38 unknown cases were identified by detecting STR and VNTR loci system The samples of putrid muscle, skeleton and tooth in 29, 6 and 2 unknown cases respectively, were identified by detecting STR loci system Only 1 sample of putrid muscle and 2 samples of skeleton tissue were failed to identification Conclusion These results suggest that the identification of unknown corpses can be distinguished using STR and VNTR loci system, especial to putrid and half-baked corpses |
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| Keywords: | PCR DNA Polymorphism VNTR STR Corpses IdentificatT |
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