FSH对仔猪睾丸支持细胞增殖和GDNF表达的调节

以原代培养的仔猪睾丸支持细胞(SC)为试验模型,研究了FSH对SC胶质细胞源性神经营养因子(GDNF)表达及其增殖的影响。结果显示,外源性添加FSH可促进GDNF蛋白表达,而且这种促进作用具有浓度和时间依赖关系,GDNF蛋白水平随着FSH浓度的增加而升高,当FSH浓度为50 ng/mL时,GDNF水平达到最高,然后逐渐降低;FSH(50 ng/mL)作用30 min,可显著促进GDNF蛋白的表达(P<0.05),当FSH作用1 h时,GDNF蛋白水平达到最高,随后逐渐降低。FSH可以时间-剂量依赖性促进SC增殖细胞核抗原(PCNA)的表达,FSH(50 ng/mL)作用30 min时,PCNA的表达显著增加(P<0.05),作用1 h达极显著水平(P<0.01),至2 h时,PCNA蛋白的表达水平达到最高,随后逐渐降低。提示,FSH可在体外条件下通过刺激SC表达GDNF进而促进其增殖。

Regulation of FSH on proliferation of piglet testis sertoli cell and expression of GDNF

Primary piglet sertoli cells(SCs) were used to analyze the effects of FSH on the proliferative potential and GDNF expression of SCs.The FSH-stimulated GDNF expression in the cultured SCs was time-and concentration-dependent,that was the expression of GDNF protein reached its highest level when the concentration of FSH was 50 ng/mL.The protein expression was increased significantly when the sti-mulating time was 30 min and reached a peak level at hour 1,then declined gradually.FSH increased expression of proliferating cell nuclear antigen(PCNA) in SCs.Expression level of PCNA protein increased significantly at 30th min and reached a peak at hour 2,then declined gradually.It was concluded that FSH was able to enhance proliferation of SCs in time-and dose-dependent manners by stimulating expression of GDNF.