| PCR技术在鸟类性别鉴定上的应用 |
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| 引用本文： | 陈武,谢淑敏,谢高基,黄勉,陈绚姣,廖明.PCR技术在鸟类性别鉴定上的应用[J].中国兽医科学,2006,36(6):485-488. |
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| 作者姓名： | 陈武 谢淑敏 谢高基 黄勉 陈绚姣 廖明 |
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| 作者单位： | 1. 广州动物园,广东,广州,510070
2. 华南农业大学,兽医学院,农业部养禽与禽病防治重点开放实验室,广东,广州,510642 |
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| 摘 要： | 根据鸟类性染色体连锁基因EE0．6在Z、W染色体上长度的不同,通过选择6对不同的引物,同时设置4种不同的反应条件,分别采用PCR技术对丹顶鹤、黑鹳、琉璃金刚鹦鹉、绯红金刚鹦鹉、鞭苔(?)鵼、戴冕鹤、火烈鸟的Z、W染色体上的EE0．6基因进行了特异性扩增。结果显示,某些引物组合能从雄鸟样品中扩增出1条片段(EE0．6Z,190 bp),而雌性鸟样品则能扩增出2务片段(EE0．6W和EE0．6Z,分别为250 bp和190 bp),但4组引物对6种鸟类EE0．6基N的扩增效果不同。证实,利用PCR技术能对鸟类进行性别鉴定。
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| 关 键 词： | PCR技术 鸟类 性别鉴定 EE0.6基因 |
| 文章编号： | 1673-4696(2006)06-0485-04 |
| 修稿时间： | 2006年2月5日 |
Application of PCR technique in sexing birds |
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| CHEN Wu,XIE Shu-min,XIE Gao-ji,HUANG Mian,CHEN Xun-jiao,LIAO Ming.Application of PCR technique in sexing birds[J].Veterinary Science in China,2006,36(6):485-488. |
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| Authors: | CHEN Wu XIE Shu-min XIE Gao-ji HUANG Mian CHEN Xun-jiao LIAO Ming |
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| Abstract: | Using the discrepancy between length of the sex-specific EE0.6 gene on the Z and W sex chromosomes of avian species, 6 different pairs of primers and 4 different reaction conditions were used to specifically amplify the EE0.6 gene on the Z and W chromosomes of Grus japonensis,Ciconia nigra,Ara ararauna,Ara macao,Ramphastos toco,Balearica pavonina,and Phoenicopterus ruberer by PCR. Under certain reation conditions, male specimens displayed one distinct amplification band (EE0.6Z,190 bp), whereas two bands(EE0.6W and EE0.6Z,250 bp and 190 bp) were amplified for the female specimens. However, amplifications in 4 different reaction conditions were different in 6 species of birds. It was concluded that the PCR technique is useful for sexing birds. |
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| Keywords: | PCR technique bird sexing EE0 6 gene |
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